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Literature summary for 2.4.1.68 extracted from

  • Miyoshi, E.; Noda, K.; Yamaguchi, Y.; Inoue, S.; Ikeda, Y.; Wang, W.; Ko, J.H.; Uozumi, N.; Li, W.; Taniguchi, N.
    The alpha1-6-fucosyltransferase gene and its biological significance (1999), Biochim. Biophys. Acta, 1473, 9-20.
    View publication on PubMed
Search for more literature for 2.4.1.68

Application

Application Comment Organism
medicine serum alpha1-6-fucosylated alpha-fetoprotein is employed for an early diagnosis of patients with hepatoma and hepatic disease Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Sus scrofa
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information gene transfection to human hepatoma and mice mutants, overexpression and effects on tumour growth Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Homo sapiens
Co2+
-
 Homo sapiens
Cu2+
-
 Homo sapiens
Cu2+
-
 Sus scrofa
additional information
-
 Homo sapiens
additional information
-
 Sus scrofa
Ni2+
-
 Homo sapiens
Ni2+
-
 Sus scrofa
Zn2+
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 GDP-L-fucose
-
 Homo sapiens
0.025
-
 N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
-
 Sus scrofa
0.028
-
 GDP-L-fucose
-
 Homo sapiens
0.029
-
 asialo-agalacto-transferrin-glycopeptide
-
 Homo sapiens
0.046
-
 GDP-L-fucose
-
 Sus scrofa
0.066
-
 asialo-agalacto-transferrin-glycopeptide
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information
-
 Homo sapiens
-
-
additional information no mRNA expression in pancreas and liver Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Homo sapiens
Mg2+ activation Homo sapiens
Mg2+ fibroblast, required Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid sequence, alignment and comparison Sus scrofa
additional information
-
 amino acid sequence, alignment and comparison Homo sapiens
additional information
-
 amino acid sequence alignment and comparison of conserved regions, consensus peptide motif Homo sapiens
additional information
-
 amino acid sequence alignment and comparison of conserved regions, consensus peptide motif Sus scrofa
34000
-
 x * 34000 + x * 39000 Homo sapiens
39000
-
 x * 34000 + x * 39000 Homo sapiens
58000
-
 1 * 58000, SDS-PAGE Homo sapiens
58000
-
 x * 58000, SDS-PAGE Sus scrofa
60000
-
 x * 60000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q9BYC5
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-
Sus scrofa P79282
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no N-glycosylation sites in predicted amino acid sequence Sus scrofa
no glycoprotein no N-glycosylation sites in predicted amino acid sequence Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
GDP-beta-L-fucose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = GDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc]-L-asparaginyl-[protein] type II transmembrane protein Homo sapiens
a
GDP-beta-L-fucose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = GDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc]-L-asparaginyl-[protein] type II transmembrane protein Sus scrofa
a
GDP-beta-L-fucose + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein] = GDP + N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->6)]-beta-D-GlcNAc]-L-asparaginyl-[protein] fucosyltransferase gene superfamily Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet thrombin-activated neutrophils release cathepsin G and elastase during coagulation of blood, which promotes the degranulation of platelets and hence the secretion of alpha-6-fucosyltransferase Homo sapiens
-
brain
-
 Rattus norvegicus
-
brain
-
 Sus scrofa
-
hepatoma cell expression in hepatoma tissues and their surrounding tissues and in tissues with chronic disease, overview Homo sapiens
-
hepatoma cell expression in hepatoma tissues and their surrounding tissues and in tissues with chronic disease, overview Rattus norvegicus
-
intestine
-
 Rattus norvegicus
-
liver
-
 Rattus norvegicus
-
liver
-
 Sus scrofa
-
liver enzyme is expressed in hepatoma tissues and their surrounding tissues with chronic disease, but not in normal liver Homo sapiens
-
lung cancer cell line
-
 Homo sapiens
-
MKN-45 cell
-
 Homo sapiens
-
serum
-
 Homo sapiens
-
serum
-
 Rattus norvegicus
-
skin fibroblast
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus
additional information
-
-
 Sus scrofa
additional information
-
 activity in hepatic disease Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + asialo-agalactotransferrin glycopeptide biantennary to tetraantennary substrates, with the triantennary substrate showing the highest activity Homo sapiens GDP + L-fucose alpha-1,6 bound to the asparagine-linked N-acetylglucosamine of the asialo-agalactotransferrin glycopeptide
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Rattus norvegicus GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
-
 Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine)
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Homo sapiens
? x * 58000, SDS-PAGE Sus scrofa
? x * 34000 + x * 39000 Homo sapiens
monomer 1 * 58000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 37
-
 Sus scrofa
30 37
-
 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10
-
 Sus scrofa
4 10
-
 Homo sapiens