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Literature summary for 2.4.1.68 extracted from

  • Koetzler, M.P.; Blank, S.; Bantleon, F.I.; Spillner, E.; Meyer, B.
    Donor substrate binding and enzymatic mechanism of human core alpha1,6-fucosyltransferase (FUT8) (2012), Biochim. Biophys. Acta, 1820, 1915-1925.
    View publication on PubMed
Search for more literature for 2.4.1.68

Application

Application Comment Organism
medicine the enzymatic product of core alpha1,6-fucosyltransferase plays a major role in a plethora of pathological conditions, e.g. in prognosis of hepatocellular carcinoma and in colon cancer Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells as soluble enzyme lacking the transmembrane region Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
co-crystallized with GDP-L-fucose Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GDP strong inhibitor Homo sapiens
GMP weak inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine pH and temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane a transmembrane protein Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 1 * 62000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9BYC5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine
-
 Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ir

Subunits

Subunits Comment Organism
monomer 1 * 62000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha1,6-fucosyltransferase
-
 Homo sapiens
alpha1-6FucT
-
 Homo sapiens
FUT8
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine pH and temperature not specified in the publication Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0036
-
 GDP pH and temperature not specified in the publication Homo sapiens
2.8
-
 GMP pH and temperature not specified in the publication Homo sapiens