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Literature summary for 2.4.1.69 extracted from

  • Scudder, P.R.; Chantler, E.N.
    Glycosyltransferases of the human cervical epithelium. I. Characterization of a beta-galactoside alpha-2-L-fucosyltransferase and the identification of a beta-N-acetylglucosaminide alpha-3-L-fucosyltransferase (1981), Biochim. Biophys. Acta, 660, 128-135.
    View publication on PubMed
Search for more literature for 2.4.1.69

Activating Compound

Activating Compound Comment Organism Structure
GTP 0.002 mM, stimulation to 160% of the control reaction Homo sapiens
Triton X-100 required Homo sapiens

General Stability

General Stability Organism
freezing and thawing has no effect Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GDP competitive Homo sapiens
GTP above 0.02 mM Homo sapiens
NEM 3 mM, almost complete inhibition Homo sapiens
PCMB
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 GDP-L-fucose
-
 Homo sapiens
8.2
-
 phenyl beta-D-galactoside
-
 Homo sapiens
33
-
 di-N-acetylchitobiose
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme requires either Mn2+ or Mg2+ for maximal activity Homo sapiens
Mn2+ enzyme requires either Mn2+ or Mg2+ for maximal activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-L-fucose + beta-D-galactosyl-R Homo sapiens the acid form of the enzyme is probably involved in glycoprotein synthesis in vivo GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cervical epithelium
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, stable for at least 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + agalacto-asialofetuin
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-agalacto-asialofetuin
-
 ?
GDP-L-fucose + asialo alpha1 acid glycoprotein
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-asialo alpha1 acid glycoprotein
-
 ?
GDP-L-fucose + asialofetuin
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-asialofetuin
-
 ?
GDP-L-fucose + beta-D-galactosyl-R
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
GDP-L-fucose + beta-D-galactosyl-R the acid form of the enzyme is probably involved in glycoprotein synthesis in vivo Homo sapiens GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
GDP-L-fucose + D-galactose
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-D-galactose
-
 ?
GDP-L-fucose + di-N-acetylchitobiose
-
 Homo sapiens GDP + ?
-
 ?
GDP-L-fucose + fetuin
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-fetuin
-
 ?
GDP-L-fucose + lacto-N-biose 1 i.e. Gal(1-3)GlcNAc Homo sapiens GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
 ?
GDP-L-fucose + lactose
-
 Homo sapiens GDP + fucosyllactose
-
 ?
GDP-L-fucose + N-acetylallolactosamine
-
 Homo sapiens GDP + ?
-
 ?
GDP-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine
-
 ?
GDP-L-fucose + phenyl-beta-D-galactoside
-
 Homo sapiens GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 at pH 6.0 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
 GDP
-
 Homo sapiens