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Literature summary for 2.4.1.69 extracted from
- Enzymatic synthesis of a blood group H-related glycosphingolipid by an alpha-fucosyltransferase from bovine spleen (1975), J. Biol. Chem., 250, 2956-2962.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| G-3634-A | activity is optimal with 5 mg/ml of the cationic detergent | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, 95% loss of activity | Bos taurus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.36 | - |
GDP-L-fucose | - |
Bos taurus | |
| 0.6 | - |
Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | |
| 1.5 | - |
Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Bos taurus | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates dialyzed enzyme | Bos taurus | |
| Mg2+ | partially prevents inactivation in presence of EDTA | Bos taurus | |
| Mn2+ | partially prevents inactivation in presence of EDTA | Bos taurus | |
| Zn2+ | stimulates dialyzed enzyme | Bos taurus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-L-fucose + beta-D-galactosyl-R | - |
Bos taurus | GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R | - |
? | |
| GDP-L-fucose + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | GDP + L-Fucalpha(1-2)Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
? | |
| GDP-L-fucose + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
Bos taurus | GDP + L-Fucalpha(1-2)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc-ceramide | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
cacodylate-HCl buffer | Bos taurus |
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