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Literature summary for 2.4.1.69 extracted from

  • Sherwood, A.L.; Stroud, M.R.; Levery, S.B.; Holmes, E.H.
    An amino acid region at the N-terminus of rat hepatoma alpha1->2 fucosyltransferase modulates enzyme activity and interaction with lipids: strong preference for glycosphingolipids containing terminal Galbeta1->3GalNAc-structures (2001), Biochemistry, 40, 5708-5719.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CHAPSO the truncated enzyme is optimally activated by CHAPSO, showing little activity in absence Rattus norvegicus
Triton CF-54 the native and the recombinant full-length enzyme display significant activity in absence of detergent or phospholipid and are optimally activated in presence of Triton CF-54 detergent Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
full length enzyme and truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information recombinant full length enzyme has a specific activity over 80fold higher than the truncated enzyme missing the first 27 amino acid residues from the N-terminus, comprising the cytoplasmic and transmembrane domains. The native and the recombinant full-length enzyme display significant activity in absence of detergent or phospholipid and are optimally activated in presence of Triton CF-54 detergent. The truncated enzyme is optimally activated by CHAPSO, showing littel activity in absence Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q10984
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Source Tissue

Source Tissue Comment Organism Textmining
H35 cell
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Rattus norvegicus
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hepatoma cell e.g. hepatoma H35 cells, the enzyme is induced during early stages of chemical hepatocarcinogenesis in parenchymal cells of Fischer 344 rats fed a diet supplemented with 0.03% N-2-acetylaminofluorene, undetectable in normal liver tissue Rattus norvegicus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-L-fucose + beta-D-galactosyl-R
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Rattus norvegicus GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
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additional information the enzyme shows a strong preference for terminal Galbeta1, 3GalNAc structures Rattus norvegicus ?
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