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Literature summary for 2.4.1.69 extracted from
- Purification and characterization of secretory-type GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase from human gastric mucosa (1995), J. Biochem., 118, 541-545.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | - |
Homo sapiens |  |
| Zn2+ | inhibition of plasma enzyme | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
GDP-L-fucose | enzyme from plasma | Homo sapiens | |
| 0.095 | - |
GDP-L-fucose | enzyme from gastric mucosa | Homo sapiens | |
| 0.095 | - |
GDP-beta-L-fucose | pH 7.2, temperature not specified in the publication | Homo sapiens | |
| 0.95 | - |
beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | pH 7.2, temperature not specified in the publication | Homo sapiens | |
| 1.1 | - |
N-acetyl-beta-lactosamine | enzyme from gastric mucosa | Homo sapiens | |
| 2.1 | - |
N-acetyl-beta-lactosamine | enzyme from plasma | Homo sapiens | |
| 3.3 | - |
phenyl-beta-D-galactoside | enzyme from plasma | Homo sapiens | |
| 4.9 | - |
lactose-N-biose I | enzyme from plasma | Homo sapiens | |
| 13 | - |
phenyl-beta-D-galactoside | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 10 mM, enzyme from gastric mucosa: 6.3% of the activation with Mn2+, enzyme from plasma: 108% of the activation with Mn2+ | Homo sapiens | |
| Ba2+ | 10 mM, 6% of the activity with Mn2+ | Homo sapiens | |
| Ca2+ | 10 mM, enzyme from gastric mucosa: 44.8% of the activation with Mn2+, enzyme from plasma: 94.2% of the activation with Mn2+ | Homo sapiens | |
| Ca2+ | 10 mM, 45% of the activity with Mn2+ | Homo sapiens | |
| Co2+ | 10 mM, enzyme from gastric mucosa: 48.6% of the activation with Mn2+, enzyme from plasma: 94.3% of the activation with Mn2+ | Homo sapiens | |
| Co2+ | 10 mM, 48% of the activity with Mn2+ | Homo sapiens | |
| Mg2+ | 10 mM, enzyme from gastric mucosa: 65.3% of the activation with Mn2+, enzyme from plasma: 98.2% of the activation with Mn2+ | Homo sapiens | |
| Mg2+ | 10 mM, 65% of the activity with Mn2+ | Homo sapiens | |
| Mn2+ | divalent cation is essential for enzyme from gastric mucosa, maximal activity with 10 mM Mn2+. Enzyme from plasma is maximally activated with 10 mM Mn2+, but activity is also observed without the cation | Homo sapiens | |
| Mn2+ | 10 mM, best activator | Homo sapiens | |
| additional information | divalent cation is absolutely required, no activation with Zn2+ | Homo sapiens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, enzyme from gastric mucosa, SDS-PAGE | Homo sapiens |
| 320000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme from gastric mucosa | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gastric mucosa | Se enzyme | Homo sapiens | - |
| mucosa | gastric mucosa | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | i.e. lacto-N-biose I | Homo sapiens | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine | - |
? | |
| GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine | i.e. N-acetyllactosamine, reaction of EC 2.4.1.344 | Homo sapiens | GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine | - |
? | |
| GDP-beta-L-fucose + lactose-N-biose I | - |
Homo sapiens | ? | - |
? | |
| GDP-L-fucose + beta-D-galactosyl-R | - |
Homo sapiens | GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R | - |
? | |
| GDP-L-fucose + lacto-N-biose 1 | - |
Homo sapiens | GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc | - |
? | |
| GDP-L-fucose + N-acetyllactosamine | - |
Homo sapiens | GDP + alpha-L-fucosyl-1,2-N-acetyllactosamine | - |
? | |
| GDP-L-fucose + phenyl-beta-D-galactoside | - |
Homo sapiens | GDP + phenyl 2-O-(alpha-L-fucopyranosyl)-beta-D-galactopyranoside | - |
? | |
| additional information | enzyme accepts both precursors of type 1 and type 2 H epitopes, catalyzing reactions of EC 2.4.1.69 and EC 2.4.1.344. The enzyme does not display alpha-(1,3)-fucosyltransferase activity or alpha-(1, 4)-fucosyl transferase activity | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, enzyme from gastric mucosa, SDS-PAGE | Homo sapiens |
| multimer | x * 65000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
enzyme from plasma and gastric mucosa | Homo sapiens |
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