Cloned (Comment) | Organism |
---|---|
expressed in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C35T | the mutation is a polymorphism in the Chinese population and does not affect its mRNA transcription, but can slightly decrease the activity of human alpha-(1,2)-fucosyltransferase in vitro | Homo sapiens |
S12F | the mutation does not significantly decrease the activity of alpha-(1,2)-fucosyltransferase and affect its kinetic property | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3984 | - |
GDP-L-fucose | Km above 0.3984 mM, isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens | |
0.3984 | - |
GDP-L-fucose | wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens | |
83.6 | - |
phenyl-beta-D-galactoside | wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens | |
125 | - |
phenyl-beta-D-galactoside | isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + phenyl-beta-D-galactoside | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-(1,2)-fucosyltransferase | - |
Homo sapiens |
FUT1 | isoform | Homo sapiens |
FUT2 | isoform | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
GDP-L-fucose | wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens | |
0.011 | - |
phenyl-beta-D-galactoside | isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens | |
0.011 | - |
phenyl-beta-D-galactoside | wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the H antigen of human blood group is synthesized by alpha-(1,2)-fucosyltransferase | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000088 | - |
phenyl-beta-D-galactoside | isoform FUT1 mutant C35T, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens | |
0.000132 | - |
phenyl-beta-D-galactoside | wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens | |
0.0183 | - |
GDP-L-fucose | wild type isoform FUT1, in 50 mM Tris-HCl (pH 7.2), at 37°C | Homo sapiens |