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Literature summary for 2.4.1.7 extracted from
- Screening for bacteria producing sucrose phosphorylase and characterization of the enzymes (1996), Biosci. Biotechnol. Biochem., 60, 319-321.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12 | - |
D-fructose | - |
Leuconostoc mesenteroides |  |
| 16 | - |
alpha-D-glucose 1-phosphate | - |
Leuconostoc mesenteroides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
SDS-PAGE, ATCC 12291 | Leuconostoc mesenteroides |
| 58000 | - |
SDS-PAGE, AKU 1102 | Leuconostoc mesenteroides |
| 58000 | - |
gel filtration, ATCC 12291 | Leuconostoc mesenteroides |
| 60000 | - |
gel filtration, AKU 1102 | Leuconostoc mesenteroides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + phosphate | Leuconostoc mesenteroides | ping-pong mechanism | alpha-D-glucose 1-phosphate + D-fructose | - |
? | |
| sucrose + phosphate | Leuconostoc mesenteroides AKU 1102 | ping-pong mechanism | alpha-D-glucose 1-phosphate + D-fructose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc mesenteroides | - |
AKU 1102 | - |
| Leuconostoc mesenteroides | - |
ATCC 12291 | - |
| Leuconostoc mesenteroides AKU 1102 | - |
AKU 1102 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leuconostoc mesenteroides |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate | ping-pong mechanism | Leuconostoc mesenteroides |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.4 | - |
purified enzyme from AKU 1102 | Leuconostoc mesenteroides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate + D-xylulose | - |
Leuconostoc mesenteroides | alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate | - |
r | |
| alpha-D-glucose 1-phosphate + D-xylulose | - |
Leuconostoc mesenteroides AKU 1102 | alpha-D-glucopyranosyl-D-xylulofuranoside + phosphate | - |
r | |
| alpha-D-glucose 1-phosphate + L-arabinose | - |
Leuconostoc mesenteroides | ? | - |
r | |
| alpha-D-glucose 1-phosphate + L-arabinose | - |
Leuconostoc mesenteroides AKU 1102 | ? | - |
r | |
| alpha-D-glucose-1-phosphate + L-sorbose | - |
Leuconostoc mesenteroides | alpha-D-glucosyl-alpha-L-sorbose + phosphate | - |
r | |
| alpha-D-glucose-1-phosphate + L-sorbose | - |
Leuconostoc mesenteroides AKU 1102 | alpha-D-glucosyl-alpha-L-sorbose + phosphate | - |
r | |
| sucrose + phosphate | ping-pong mechanism | Leuconostoc mesenteroides | alpha-D-glucose 1-phosphate + D-fructose | - |
? | |
| sucrose + phosphate | ping-pong mechanism | Leuconostoc mesenteroides | alpha-D-glucose 1-phosphate + D-fructose | - |
r | |
| sucrose + phosphate | ping-pong mechanism | Leuconostoc mesenteroides AKU 1102 | alpha-D-glucose 1-phosphate + D-fructose | - |
? | |
| sucrose + phosphate | ping-pong mechanism | Leuconostoc mesenteroides AKU 1102 | alpha-D-glucose 1-phosphate + D-fructose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | gel filtration, SDS-PAGE | Leuconostoc mesenteroides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 40 | reverse reaction | Leuconostoc mesenteroides |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
rapid loss of activity above 40°C | Leuconostoc mesenteroides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | reverse reaction | Leuconostoc mesenteroides |
| 7 | 7.5 | forward reaction | Leuconostoc mesenteroides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | stable at 30°C | Leuconostoc mesenteroides |
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