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Literature summary for 2.4.1.7 extracted from

  • Sprogoe, D.; van den Broek, L.A.; Mirza, O.; Kastrup, J.S.; Voragen, A.G.; Gajhede, M.; Skov, L.K.
    Crystal structure of sucrose phosphorylase from Bifidobacterium adolescentis (2004), Biochemistry, 43, 1156-1162.
    View publication on PubMed
Search for more literature for 2.4.1.7

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme, hanging drop vapour diffusion method, 0.0025 ml protein solution, containing 0.5-1.0 mg/ml protein, 10 mM Tris-HCl, pH 7.1, is mixed with eual volume of precipitant solution containing 27% w/v PEG 4000, 0.1 M Tris-HCl, pH 8.5, and 0.1 sodium acetate, at 25°C, 3-14 days, X-ray diffraction structure determination and analysis at 1.77 A resolution Bifidobacterium adolescentis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21400
-
 2 * 21400, dynamic light scattering Bifidobacterium adolescentis
56190
-
 mass spectrometry, gel filtration Bifidobacterium adolescentis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + phosphate Bifidobacterium adolescentis
-
 beta-D-fructose + alpha-D-glucose 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Bifidobacterium adolescentis Q84HQ2 gene BiSP
-

Reaction

Reaction Comment Organism Reaction ID
sucrose + phosphate = D-fructose + alpha-D-glucose 1-phosphate reaction mechanism, substrate access channel structure, catalytic active site residues are Asp192 and Glu232 Bifidobacterium adolescentis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + phosphate
-
 Bifidobacterium adolescentis beta-D-fructose + alpha-D-glucose 1-phosphate
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 21400, dynamic light scattering Bifidobacterium adolescentis
More an enzyme monomer consists of 4 domains: A, B, B', and C, domain A comprises the (beta/alpha)8-barrel including the active site, domain B' is involved in modulation of substrate acces via the substrate channel Bifidobacterium adolescentis

Synonyms

Synonyms Comment Organism
BiSP
-
 Bifidobacterium adolescentis
More the enzyme belongs to the glycoside hydrolase family 13 Bifidobacterium adolescentis