Protein Variants | Comment | Organism |
---|---|---|
D196N/E237Q | the mutation affects the the stereoselectivity of the reaction | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, ping-pong kinetics | Leuconostoc mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-fluoride + phosphate | Leuconostoc mesenteroides | - |
fluoride + alpha-D-glucose 1-phosphate | - |
r | |
sucrose + phosphate | Leuconostoc mesenteroides | - |
D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-fluoride + phosphate | - |
Leuconostoc mesenteroides | fluoride + alpha-D-glucose 1-phosphate | - |
r | |
alpha-D-glucose 1-fluoride + phosphate | mechanisms for wild-type sucrose phosphorylase and doubly mutated variants, overview | Leuconostoc mesenteroides | fluoride + alpha-D-glucose 1-phosphate | - |
r | |
alpha-D-glucose 1-phosphate + arsenate | - |
Leuconostoc mesenteroides | alpha-D-glucose 1-arsenate + phosphate | - |
? | |
additional information | alpha-retaining glucosyl transfer through front-side bimolecular nucleophilic substitution | Leuconostoc mesenteroides | ? | - |
? | |
sucrose + phosphate | - |
Leuconostoc mesenteroides | D-fructose + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | model of a ternary complex between mutated sucrose phosphorylase, alpha-gklucosyl 1-fluorid, and phosphate, overview | Leuconostoc mesenteroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
alpha-D-glucose 1-phosphate | arsenolysis of alpha-D-glucose 1-phosphate, mutant D196N/E237Q | Leuconostoc mesenteroides | |
170 | - |
alpha-D-glucose 1-phosphate | arsenolysis of alpha-D-glucose 1-phosphate, wild-type enzyme | Leuconostoc mesenteroides |