Literature summary for 2.4.1.7 extracted from

Kraus, M.; Grimm, C.; Seibel, J.
Redesign of the active site of sucrose phosphorylase through a clash-induced cascade of loop shifts (2016), ChemBioChem, 17, 33-36.

Search for more literature for 2.4.1.7

Crystallization (Commentary)

Crystallization (Comment)	Organism
recombinant enzyme mutant Q345F, in the presence of sucrose, and in complex with glucose, X-ray diffraction structure determination and analysis at 2.7 A resolution, PDB ID 5C8B	Bifidobacterium adolescentis

Protein Variants

Protein Variants	Comment	Organism
additional information	redesign of the active site of sucrose phosphorylase through a clash-induced cascade of loop shifts	Bifidobacterium adolescentis
Q345F	site-directed mutagenesis, the mutation allows efficient glucosylation of resveratrol, (+)-catechin and (-)-epicatechin in yields of up to 97% whereas the wild-type enzyme favours sucrose hydrolysis. The crystal structure of the variant reveals a widened access channel with a hydrophobic aromatic surface that is likely to contribute to the improved activity towards aromatic acceptors. The generation of this channel can be explained in terms of a cascade of structural changes arising from the Q345F exchange, structural changes in the active site of BaSP Q345F, modeling, overview	Bifidobacterium adolescentis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + phosphate	Bifidobacterium adolescentis	-	D-fructose + alpha-D-glucose 1-phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Bifidobacterium adolescentis	Q84HQ2	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the wild-type enzyme shows poor activity with flavonoids or stilbenoids, e.g. resveratrol, (+)-catechin and (-)-epicatechin, while the compounds are substrates of the enzyme mutant Q345F	Bifidobacterium adolescentis	?	-	?
sucrose + (+)-catechin	activity of enzyme mutant Q345F	Bifidobacterium adolescentis	D-fructose + (+)-catechin 3'-O-alpha-D-glucoside + (+)-catechin 3',5-O-alpha-D-diglucoside	-	?
sucrose + (-)-epicatechin	activity of enzyme mutant Q345F	Bifidobacterium adolescentis	D-fructose + (-)-epicatechin 3'-O-alpha-D-glucoside + (-)-epicatechin 5-O-alpha-D-glucoside + (-)-epicatechin 3',5-O-alpha-D-diglucoside	-	?
sucrose + phosphate	-	Bifidobacterium adolescentis	D-fructose + alpha-D-glucose 1-phosphate	-	r
sucrose + phosphate	activity of wild-type enzyme and mutant Q345F	Bifidobacterium adolescentis	D-fructose + alpha-D-glucose 1-phosphate	-	r
sucrose + resveratrol	activity of enzyme mutant Q345F	Bifidobacterium adolescentis	D-fructose + resveratrol 3-O-alpha-D-glucoside	-	?

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Release 2023.1 (January 2023)