Cloned (Comment) | Organism |
---|---|
expression of the enzyme in a Saccharomyces cerevisiae knockout strain showing no maltose-transport activity and a very low residual maltase activity, co-expression with the MAL11 maltose-transporter gene and Lactococcus lactis beta-phosphoglucomutase | Fructilactobacillus sanfranciscensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Replacing maltose hydrolysis with phosphorolysis, by co-expression of the maltose phosphorylase with MAL11 maltose-transporter gene and Lactococcus lactis beta-phosphoglucomutase, in a Saccharomyces cerevisiae straain IMK289, a maltose transporter and maltase knockout strain, increases the anaerobic biomass yield on maltose in anaerobic maltose-limited chemostat cultures by 26%, thus demonstrating the potential of phosphorolysis to improve the free-energy conservation of disaccharide metabolism in industrial microorganisms, phenotype, overview | Fructilactobacillus sanfranciscensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + phosphate | Fructilactobacillus sanfranciscensis | - |
D-glucose + beta-D-glucose 1-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fructilactobacillus sanfranciscensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + phosphate | - |
Fructilactobacillus sanfranciscensis | D-glucose + beta-D-glucose 1-phosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Fructilactobacillus sanfranciscensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | - |
assay at | Fructilactobacillus sanfranciscensis |
General Information | Comment | Organism |
---|---|---|
additional information | maltose phosphorylase is a disaccharide phosphorylase | Fructilactobacillus sanfranciscensis |