General Stability | Organism |
---|---|
NAD+ stabilizes | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CDP-glucose | slight inhibition | Mus musculus | |
CHAPS | i.e. 3-[(3-cholamidopropyl)dimethylammonio]-1propanesulfonate | Mus musculus | |
Cu2+ | - |
Mus musculus | |
Fe2+ | - |
Mus musculus | |
Fe3+ | - |
Mus musculus | |
Tris | at high concentration | Mus musculus | |
Zn2+ | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Mus musculus | |
Mg2+ | stimulates | Mus musculus | |
Mn2+ | stimulates | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay method development | Mus musculus |
Storage Stability | Organism |
---|---|
-70°C, enzyme in intact kidneys is stable for at least 90 days | Mus musculus |
4°C, enzyme in intact kidneys, 53% loss of activity after overnight storage | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | i.e. ceramide | Mus musculus | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Mus musculus |