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Literature summary for 2.4.1.80 extracted from

  • Matsuo, N.; Nomura, T.; Imokawa, G.
    A rapid and simple assay method for UDP-glucose:ceramide glucosyltransferase (1992), Biochim. Biophys. Acta, 1116, 97-103.
    View publication on PubMed
Search for more literature for 2.4.1.80

Activating Compound

Activating Compound Comment Organism Structure
CHAPS i.e. 3-[(3-cholamidopropyl)dimethylamonio]-1propanesulfonate Rattus norvegicus
detergent activity is reduced by most of the detergents tested, only CHAPS and CHAPSO at concentration of 1% w/v stimulate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Rattus norvegicus
Zn2+
-
 Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 UDP-glucose
-
 Rattus norvegicus
0.292
-
 ceramide
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Rattus norvegicus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ activates Rattus norvegicus
Mg2+ stimulates Rattus norvegicus
Mn2+ stimulates Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 assay method development Rattus norvegicus
0.00031
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4 6.5 MES or Tris-maleate buffer Rattus norvegicus