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Literature summary for 2.4.1.82 extracted from

  • Schneider, T.; Keller, F.
    Raffinose in chloroplasts is synthesized in the cytosol and transported across the chloroplast envelope (2009), Plant Cell Physiol., 50, 2174-2182.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Spinacia oleracea 5829
-
cytosol
-
Arabidopsis thaliana 5829
-
cytosol
-
Ajuga reptans 5829
-
protoplast
-
Spinacia oleracea
-
-
protoplast
-
Arabidopsis thaliana
-
-
protoplast
-
Ajuga reptans
-
-

Organism

Organism UniProt Comment Textmining
Ajuga reptans
-
-
-
Ajuga reptans
-
plants are cold-treated
-
Arabidopsis thaliana
-
-
-
Arabidopsis thaliana
-
plants are cold-treated
-
Spinacia oleracea
-
-
-
Spinacia oleracea
-
plants are cold-treated
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-
leaf
-
Arabidopsis thaliana
-
leaf
-
Ajuga reptans
-
mesophyll
-
Spinacia oleracea
-
mesophyll
-
Arabidopsis thaliana
-
mesophyll
-
Ajuga reptans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
no enzyme activity is found in chloroplasts Spinacia oleracea
additional information
-
no enzyme activity is found in chloroplasts Arabidopsis thaliana
additional information
-
no enzyme activity is found in chloroplasts Ajuga reptans
0.00138
-
protoplast Ajuga reptans
0.00258
-
protoplast Arabidopsis thaliana
0.00396
-
protoplast Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol + raffinose
-
Spinacia oleracea 1-alpha-D-galactosyl-1D-myo-inositol + sucrose
-
r
myo-inositol + raffinose
-
Arabidopsis thaliana 1-alpha-D-galactosyl-1D-myo-inositol + sucrose
-
r
myo-inositol + raffinose
-
Ajuga reptans 1-alpha-D-galactosyl-1D-myo-inositol + sucrose
-
r

Synonyms

Synonyms Comment Organism
raffinose synthase
-
Spinacia oleracea
raffinose synthase
-
Arabidopsis thaliana
raffinose synthase
-
Ajuga reptans
RafS
-
Spinacia oleracea
RafS
-
Arabidopsis thaliana
RafS
-
Ajuga reptans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
assay at, RafS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Spinacia oleracea
6.5
-
assay at, RafS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Arabidopsis thaliana
6.5
-
assay at, RafS enzyme activity shows neutral pH optima, corresponding to the pH of the cytosol Ajuga reptans

General Information

General Information Comment Organism
physiological function enzyme is involved in stress resistance Spinacia oleracea
physiological function enzyme is involved in stress resistance Arabidopsis thaliana
physiological function enzyme is involved in stress resistance Ajuga reptans