Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | absolute requirement, maximal activity at 0.5-1.0% v/v | Mus musculus |
General Stability | Organism |
---|---|
detergent: rapid and irreversible inactivation in absence of detergent, more than 50% loss of activity after 4-6 weeks | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
beta-D-Gal(1-4)-D-GlcNAc(1-2)-[beta-D-Gal(1-4)beta-D-GlcNAc(1-6)]-D-Man | - |
Mus musculus | |
0.25 | - |
beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man | - |
Mus musculus | |
0.31 | - |
beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man | - |
Mus musculus | |
1.15 | - |
beta-D-Gal(1-4)-D-GlcNAc | - |
Mus musculus | |
12.6 | - |
UDP-galactose | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mus musculus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
x * 80000, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ehrlich ascites carcinoma cells | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.001 | - |
membrane fraction | Mus musculus |
265 | - |
purified enzyme | Mus musculus |
Storage Stability | Organism |
---|---|
-20°C, 20 mM MES, pH 6.2, 50 mM NaCl, detergent, glycerol, stable for several months | Mus musculus |
0°C, more than 50% loss of activity after 4-6 weeks | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | acceptor substrate specificity | Mus musculus | ? | - |
? | |
UDP-galactose + beta-D-Gal(1-4)-D-GlcNAc(1-2)-[beta-D-Gal(1-4)beta-D-GlcNAc(1-6)]-D-Man | - |
Mus musculus | ? | - |
? | |
UDP-galactose + beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man | - |
Mus musculus | UDP + alpha-D-Gal(1-3)beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man | - |
? | |
UDP-galactose + beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man | - |
Mus musculus | UDP + alpha-D-Gal(1-3)beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man | - |
? | |
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosamine | - |
Mus musculus | UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine | - |
? | |
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | not: alpha-L-Fuc(1-2)beta-D-Gal(1-4)-D-GlcNAc | Mus musculus | UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | beta-D-Gal(1-4)-[alpha-L-Fuc(1-3)]-D-GlcNAc | Mus musculus | UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | very poor acceptor: lactose, beta-D-Gal(1-3)-D-GlcNAc | Mus musculus | UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-galactose + lactose | very poor acceptor | Mus musculus | UDP + alpha-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-glucose | - |
? | |
UDP-galactose + N-acetyllactosamine | oligosaccharides, glycoproteins and glycosaminoglycans containing the terminal nonreducing N-acetyllactosamine unit all serve as acceptors | Mus musculus | UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 80000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.3 | 7 | pH 5.3: about 45% of activity maximum, pH 7.0: about 30% of activity maximum | Mus musculus |