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Literature summary for 2.4.1.87 extracted from

  • Elices, M.J.; Blake, D.A.; Goldstein, I.J.
    Purification and characterization of a UDP-Gal:beta-D-Gal(1,4)-D-GlcNAc alpha(1,3)-galactosyltransferase from Ehrlich ascites tumor cells (1986), J. Biol. Chem., 261, 6064-6072.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 absolute requirement, maximal activity at 0.5-1.0% v/v Mus musculus

General Stability

General Stability Organism
detergent: rapid and irreversible inactivation in absence of detergent, more than 50% loss of activity after 4-6 weeks Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
beta-D-Gal(1-4)-D-GlcNAc(1-2)-[beta-D-Gal(1-4)beta-D-GlcNAc(1-6)]-D-Man
-
Mus musculus
0.25
-
beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man
-
Mus musculus
0.31
-
beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man
-
Mus musculus
1.15
-
beta-D-Gal(1-4)-D-GlcNAc
-
Mus musculus
12.6
-
UDP-galactose
-
Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
x * 80000, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ehrlich ascites carcinoma cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
Ehrlich ascites carcinoma cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
membrane fraction Mus musculus
265
-
purified enzyme Mus musculus

Storage Stability

Storage Stability Organism
-20°C, 20 mM MES, pH 6.2, 50 mM NaCl, detergent, glycerol, stable for several months Mus musculus
0°C, more than 50% loss of activity after 4-6 weeks Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information acceptor substrate specificity Mus musculus ?
-
?
UDP-galactose + beta-D-Gal(1-4)-D-GlcNAc(1-2)-[beta-D-Gal(1-4)beta-D-GlcNAc(1-6)]-D-Man
-
Mus musculus ?
-
?
UDP-galactose + beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man
-
Mus musculus UDP + alpha-D-Gal(1-3)beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man
-
?
UDP-galactose + beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man
-
Mus musculus UDP + alpha-D-Gal(1-3)beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man
-
?
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
?
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R not: alpha-L-Fuc(1-2)beta-D-Gal(1-4)-D-GlcNAc Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
?
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R beta-D-Gal(1-4)-[alpha-L-Fuc(1-3)]-D-GlcNAc Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
?
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R very poor acceptor: lactose, beta-D-Gal(1-3)-D-GlcNAc Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
?
UDP-galactose + lactose very poor acceptor Mus musculus UDP + alpha-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-glucose
-
?
UDP-galactose + N-acetyllactosamine oligosaccharides, glycoproteins and glycosaminoglycans containing the terminal nonreducing N-acetyllactosamine unit all serve as acceptors Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
-
Mus musculus

pH Range

pH Minimum pH Maximum Comment Organism
5.3 7 pH 5.3: about 45% of activity maximum, pH 7.0: about 30% of activity maximum Mus musculus