General Stability | Organism |
---|---|
glycerol, 50%, stabilizes during storage | Canis lupus familiaris |
repeated freeze-thawing inactivates, UDP and glycerol protect | Canis lupus familiaris |
UDP, 0.1 mM, stabilizes during storage | Canis lupus familiaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | in the presence of Mn2+ | Canis lupus familiaris | |
Cu2+ | in the presence of Mn2+ | Canis lupus familiaris | |
Ni2+ | in the presence of Mn2+ | Canis lupus familiaris | |
UDP | competitive to UDP-N-acetylgalactosamine, non-competitive to globoside | Canis lupus familiaris | |
Zn2+ | in the presence of Mn2+ | Canis lupus familiaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00156 | - |
globoside | - |
Canis lupus familiaris | |
0.01 | - |
UDP-N-acetylgalactosamine | - |
Canis lupus familiaris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Canis lupus familiaris | 16020 | - |
microsome | - |
Canis lupus familiaris | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mn2+ with 20% efficiency, inhibits in the presence of Mn2+ | Canis lupus familiaris | |
Cu2+ | can replace Mn2+ with 31% efficiency, inhibits in the presence of Mn2+ | Canis lupus familiaris | |
Fe2+ | can replace Mn2+ with 76% efficiency | Canis lupus familiaris | |
Mg2+ | stimulation, only in the presence of Mn2+ | Canis lupus familiaris | |
Mn2+ | requirement | Canis lupus familiaris | |
Mn2+ | Km-value 0.45 mM | Canis lupus familiaris | |
additional information | no activation by Ni2+ | Canis lupus familiaris | |
Zn2+ | can replace Mn2+ with 83% efficiency | Canis lupus familiaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
1 * 56000 + 1 * 60000, SDS-PAGE | Canis lupus familiaris |
60000 | - |
1 * 56000 + 1 * 60000, SDS-PAGE | Canis lupus familiaris |
120000 | - |
gel filtration | Canis lupus familiaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | Canis lupus familiaris | - |
UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | i.e. Forssman hapten | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
mongrel dog, adult | - |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Canis lupus familiaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
spleen | - |
Canis lupus familiaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000059 | - |
purified enzyme | Canis lupus familiaris |
Storage Stability | Organism |
---|---|
-80°C, 0.1 mg/ml enzyme in 25 mM sodium cacodylate buffer with 1% Triton X-100, at least 6 months | Canis lupus familiaris |
4°C, 0.1 mg/ml enzyme in 25 mM sodium cacodylate buffer with 1% Triton X-100, 40% loss of activity within 3 months | Canis lupus familiaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no substrates: oligosaccharide of blood type H substance, asialo-GM1, asialo-GM2 and GD1a, galactosyl-alpha-1,4-galactosyl-beta-1,4-glucosylceramide, UDP, UDP-galactose, 2'-fucosyllactose, H blood group glycoprotein, bovine submaxillary deglycosylated mucin, GM1-3, glucosyl-beta-1,1-ceramide, galactosyl-beta-1,1-ceramide, galactosyl-beta-1,4-glucosylceramide | Canis lupus familiaris | ? | - |
? | |
UDP-N-acetyl-alpha-D-galactosamine + globoside | - |
Canis lupus familiaris | UDP + N-acetyl-D-galactosaminyl-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | - |
? | |
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | - |
Canis lupus familiaris | UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | i.e. Forssman hapten | ? | |
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | i.e. globoside, catalyzes the transfer of N-acetylgalactosamine in alpha-1,3-linkage to globoside | Canis lupus familiaris | UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | i.e. Forssman hapten | ir | |
UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | general structure of preferred substrates: N-acetylgalactosaminyl-beta-1,3-galactosyl-O-R | Canis lupus familiaris | UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide | i.e. Forssman hapten | ir | |
UDP-N-acetyl-D-galactosamine + N-acetylgalactosaminyl-1,3-beta-galactosyl-alpha-1,3-galactosyl-beta-1,4-glucosylceramide | - |
Canis lupus familiaris | UDP + N-acetyl-D-galactosamine-1,3-N-acetylgalactosaminyl-1,3-beta-galactosyl-alpha-1,3-galactosyl-beta-1,4-glucosylceramide | - |
? | |
UDP-N-acetyl-D-galactosamine + oligosaccharide of globoside | - |
Canis lupus familiaris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 56000 + 1 * 60000, SDS-PAGE | Canis lupus familiaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
- |
Canis lupus familiaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.9 | - |
Canis lupus familiaris |