Literature summary for 2.4.1.9 extracted from

Olivares-Illana, V.; Lopez-Munguia, A.; Olvera, C.
Molecular characterization of inulosucrase from Leuconostoc citreum: a fructosyltransferase within a glucosyltransferase (2003), J. Bacteriol., 185, 3606-3612.

Search for more literature for 2.4.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the full-length enzyme and two C-terminally truncated enzyme forms in Escherichia coli. Natural chimeric enzyme with three domains: the first and third with high identity with alternansucrase, the second domain, which is the catalytic one has similarity with levansucrase and inulosucrase	Leuconostoc citreum

Protein Variants

Protein Variants	Comment	Organism
additional information	when the C-terminal domain is deleted the resulting enzyme is more active and more hydrolytic than the wild-type enzyme but is more sensitive to inactivation at 40°C	Leuconostoc citreum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup	Leuconostoc citreum	-	alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup	-	?

Organism

Organism	UniProt	Comment	Textmining
Leuconostoc citreum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup	-	Leuconostoc citreum	alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup	-	?
sucrose + [(beta-D-Fruf-(2-1))n]-alpha-D-Glup	wild-type and recombinant enzyme produce inulin from sucose	Leuconostoc citreum	alpha-D-glucose + [(beta-D-Fruf-(2-1))n+1]-alpha-D-Glup	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
40	-	half-life of wild-type enzyme: 70 min, half-life of the two C-terminally truncated mutant enzymes is 20 min and 12 min	Leuconostoc citreum

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Release 2023.1 (January 2023)