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Literature summary for 2.4.1.9 extracted from
- Biochemical properties of inulosucrase from Leuconostoc citreum CW28 used for inulin synthesis (2004), Biocatal. Biotransform., 22, 275-281.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | enzyme is used for inulin synthesis | Leuconostoc citreum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 11.4 | - |
sucrose | hydrolytic activity, measurement of beta-D-fructose, pH 6.5, 30°C | Leuconostoc citreum |  |
| 35.2 | - |
sucrose | overall activity, measurement of alpha-D-glucose, pH 6.5, 30°C | Leuconostoc citreum | |
| 99.2 | - |
sucrose | transferase activity, measurement of alpha-D-glucose minus D-fructose, pH 6.5, 30°C | Leuconostoc citreum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 165000 | - |
x * 165000, SDS-PAGE | Leuconostoc citreum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,1-beta-D-fructosyl)n | Leuconostoc citreum | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,1-beta-D-fructosyl)n | Leuconostoc citreum CW28 | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri 121 | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leuconostoc citreum | - |
isolated from Pozol, a traditional lactic acid fermented beverage of Mayan origin produced from lime treated corn in the south of Mexico | - |
| Leuconostoc citreum CW28 | - |
isolated from Pozol, a traditional lactic acid fermented beverage of Mayan origin produced from lime treated corn in the south of Mexico | - |
| Limosilactobacillus reuteri | - |
- |
- |
| Limosilactobacillus reuteri 121 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,1-beta-D-fructosyl)n | - |
Leuconostoc citreum | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
| sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Leuconostoc citreum | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin, determination of inulin product chain length, overview | ? | |
| sucrose + (2,1-beta-D-fructosyl)n | - |
Leuconostoc citreum CW28 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Leuconostoc citreum CW28 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin, determination of inulin product chain length, overview | ? | |
| sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri 121 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
| sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
| sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri | alpha-D-glucose + beta-D-fructose | - |
? | |
| sucrose + H2O | hydrolytic activity | Leuconostoc citreum | alpha-D-glucose + beta-D-fructose | - |
? | |
| sucrose + H2O | hydrolytic activity | Leuconostoc citreum CW28 | alpha-D-glucose + beta-D-fructose | - |
? | |
| sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + beta-D-fructose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 165000, SDS-PAGE | Leuconostoc citreum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IS | - |
Limosilactobacillus reuteri |
| IS | - |
Leuconostoc citreum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Limosilactobacillus reuteri |
| 30 | - |
assay at | Leuconostoc citreum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10 | - |
sucrose | hydrolytic activity, measurement of beta-D-fructose, pH 6.5, 30°C | Limosilactobacillus reuteri | |
| 25.5 | - |
sucrose | hydrolytic activity, measurement of beta-D-fructose, pH 6.5, 30°C | Leuconostoc citreum | |
| 43.6 | - |
sucrose | overall activity, measurement of alpha-D-glucose, pH 6.5, 30°C | Limosilactobacillus reuteri | |
| 55.2 | - |
sucrose | transferase activity, measurement of alpha-D-glucose minus beta-D-fructose, pH 6.5, 30°C | Limosilactobacillus reuteri | |
| 63.5 | - |
sucrose | transferase activity, measurement of alpha-D-glucose minus beta-D-fructose, pH 6.5, 30°C | Leuconostoc citreum | |
| 76.7 | - |
sucrose | overall activity, measurement of alpha-D-glucose, pH 6.5, 30°C | Leuconostoc citreum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Limosilactobacillus reuteri |
| 6.5 | - |
assay at | Leuconostoc citreum |
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