Cloned (Comment) | Organism |
---|---|
expression of Myc/His-tagged wild-type and mutant enzymes in Escherichia coli | Limosilactobacillus reuteri |
Protein Variants | Comment | Organism |
---|---|---|
D520A | site-directed mutagenesis, reduced activity in absence of Ca2+ at 30°C, reduction in affinity for Ca2+ at higher temperatures, overview | Limosilactobacillus reuteri |
D520N | site-directed mutagenesis, mutant enzyme is nearly inactive in presence of 1 mM Ca2+ and completely inactive in absence of Ca2+ | Limosilactobacillus reuteri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 40% inhibition at 30-37°C, no inhibition at room temperature, inhibitory effect increases with temperature | Limosilactobacillus reuteri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | important structural role, Asp520 is important for Ca2+-binding, binding site structure, determination of Ca2+-binding affinity | Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri 121 | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | Q8GP32 | gene ftf | - |
Limosilactobacillus reuteri 121 | Q8GP32 | gene ftf | - |
Purification (Comment) | Organism |
---|---|
recombinant Myc/His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography | Limosilactobacillus reuteri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
below, purified recombinant mutant D520A, 50°C, without Ca2+ | Limosilactobacillus reuteri |
1 | - |
purified recombinant mutant D520N, 50°C, 1 mM Ca2+ | Limosilactobacillus reuteri |
3.6 | - |
purified recombinant mutant D520A, 50°C, 1 mM Ca2+ | Limosilactobacillus reuteri |
180 | - |
purified recombinant wild-type enzyme, 50°C, 1 mM Ca2+ | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri 121 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri | alpha-D-glucose + beta-D-fructose | - |
? | |
sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + beta-D-fructose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fructosyltransferase | - |
Limosilactobacillus reuteri |
FTF | - |
Limosilactobacillus reuteri |
More | enzyme belongs to the family 68 of glucoside hydrolases | Limosilactobacillus reuteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in presence of EDTA | Limosilactobacillus reuteri |
55 | - |
in presence of Ca2+ | Limosilactobacillus reuteri |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 55 | in presence of Ca2+ at 1 mM: optimal activity at 55°C, in absence of Ca2+ and EDTA: decrease in activity above 45°C, completely inactive at 55°C, overview | Limosilactobacillus reuteri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Ca2+ stabilizes the enzyme | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
assay at | Limosilactobacillus reuteri |