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Literature summary for 2.4.1.9 extracted from

  • Ozimek, L.K.; Euverink, G.J.; van der Maarel, M.J.; Dijkhuizen, L.
    Mutational analysis of the role of calcium ions in the Lactobacillus reuteri strain 121 fructosyltransferase (levansucrase and inulosucrase) enzymes (2005), FEBS Lett., 579, 1124-1128.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of Myc/His-tagged wild-type and mutant enzymes in Escherichia coli Limosilactobacillus reuteri

Protein Variants

Protein Variants Comment Organism
D520A site-directed mutagenesis, reduced activity in absence of Ca2+ at 30°C, reduction in affinity for Ca2+ at higher temperatures, overview Limosilactobacillus reuteri
D520N site-directed mutagenesis, mutant enzyme is nearly inactive in presence of 1 mM Ca2+ and completely inactive in absence of Ca2+ Limosilactobacillus reuteri

Inhibitors

Inhibitors Comment Organism Structure
EDTA 40% inhibition at 30-37°C, no inhibition at room temperature, inhibitory effect increases with temperature Limosilactobacillus reuteri

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ important structural role, Asp520 is important for Ca2+-binding, binding site structure, determination of Ca2+-binding affinity Limosilactobacillus reuteri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (2,1-beta-D-fructosyl)n Limosilactobacillus reuteri
-
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
?
sucrose + (2,1-beta-D-fructosyl)n Limosilactobacillus reuteri 121
-
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
?

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri Q8GP32 gene ftf
-
Limosilactobacillus reuteri 121 Q8GP32 gene ftf
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Myc/His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Limosilactobacillus reuteri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
below, purified recombinant mutant D520A, 50°C, without Ca2+ Limosilactobacillus reuteri
1
-
purified recombinant mutant D520N, 50°C, 1 mM Ca2+ Limosilactobacillus reuteri
3.6
-
purified recombinant mutant D520A, 50°C, 1 mM Ca2+ Limosilactobacillus reuteri
180
-
purified recombinant wild-type enzyme, 50°C, 1 mM Ca2+ Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,1-beta-D-fructosyl)n
-
Limosilactobacillus reuteri alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
?
sucrose + (2,1-beta-D-fructosyl)n transferase activity Limosilactobacillus reuteri alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin ?
sucrose + (2,1-beta-D-fructosyl)n
-
Limosilactobacillus reuteri 121 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
?
sucrose + (2,1-beta-D-fructosyl)n transferase activity Limosilactobacillus reuteri 121 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 i.e. inulin ?
sucrose + H2O hydrolytic activity Limosilactobacillus reuteri alpha-D-glucose + beta-D-fructose
-
?
sucrose + H2O hydrolytic activity Limosilactobacillus reuteri 121 alpha-D-glucose + beta-D-fructose
-
?

Synonyms

Synonyms Comment Organism
fructosyltransferase
-
Limosilactobacillus reuteri
FTF
-
Limosilactobacillus reuteri
More enzyme belongs to the family 68 of glucoside hydrolases Limosilactobacillus reuteri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
in presence of EDTA Limosilactobacillus reuteri
55
-
in presence of Ca2+ Limosilactobacillus reuteri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
22 55 in presence of Ca2+ at 1 mM: optimal activity at 55°C, in absence of Ca2+ and EDTA: decrease in activity above 45°C, completely inactive at 55°C, overview Limosilactobacillus reuteri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
Ca2+ stabilizes the enzyme Limosilactobacillus reuteri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
assay at Limosilactobacillus reuteri