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Literature summary for 2.4.1.9 extracted from
- The probiotic Lactobacillus johnsonii NCC 533 produces high-molecular-mass inulin from sucrose by using an inulosucrase enzyme (2008), Appl. Environ. Microbiol., 74, 3426-3433.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Lactobacillus johnsonii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.7 mM, 5% residual activity. Addition of Ca2+ restores activity | Lactobacillus johnsonii |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | transfructosylation reaction does not obey Michaelis-Menten kinetics | Lactobacillus johnsonii |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | required for activity and stability | Lactobacillus johnsonii | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus johnsonii | Q74K42 | - |
- |
| Lactobacillus johnsonii NCC 533 | Q74K42 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Lactobacillus johnsonii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 27 | - |
hydrolytic activity, 55°C, pH 7.0 | Lactobacillus johnsonii |
| 178 | - |
hydrolytic activity, 37°C, pH 7.0 | Lactobacillus johnsonii |
| 187 | - |
transglycosylation activity, 37°C, pH 7.0 | Lactobacillus johnsonii |
| 365 | - |
total activity, 37°C, pH 7.0 | Lactobacillus johnsonii |
| 698 | - |
transglycosylation activity, 55°C, pH 7.0 | Lactobacillus johnsonii |
| 719 | - |
total activity, 55°C, pH 7.0 | Lactobacillus johnsonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C | Lactobacillus johnsonii | alpha-D-glucose + beta-D-fructose | - |
? | |
| sucrose + H2O | hydrolytic activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C | Lactobacillus johnsonii NCC 533 | alpha-D-glucose + beta-D-fructose | - |
? | |
| sucrose + sucrose | transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C | Lactobacillus johnsonii | [(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose | - |
? | |
| sucrose + sucrose | transglycosylation activity, ratio of transglycosylation to hydrolysis activities is almost 1:1 at 37°C but increases about 26fold at 55°C | Lactobacillus johnsonii NCC 533 | [(beta-D-fructose-(2-1))n]-alpha-D-glucose + alpha-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| InuJ | - |
Lactobacillus johnsonii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Lactobacillus johnsonii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
sharp decrease in activity above | Lactobacillus johnsonii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | more than 85% of maximum activity | Lactobacillus johnsonii |
| 7.5 | - |
sharp decrease in activity above | Lactobacillus johnsonii |
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Release 2023.1 (January 2023)
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