Cloned (Comment) | Organism |
---|---|
the truncated mutant IslA4 is expressed in Escherichia coli BL21(DE3) cells | Leuconostoc citreum |
Protein Variants | Comment | Organism |
---|---|---|
L499F | the mutation reduces the affinity for sucrose 3fold | Leuconostoc citreum |
additional information | IslA4 is a truncated form of inulosucrase that contains only the catalytic domain, this truncated form is more hydrolytic than the wild type enzyme and produces both high-molecular-weight inulin and fructooligosaccharides | Leuconostoc citreum |
R618K | mutant shows decreased activity compared to the wild type enzyme | Leuconostoc citreum |
R696K | the mutant only produces inulin | Leuconostoc citreum |
S425A | the mutant displays complex kinetic behavior in which the transfructosylation rate is described by first-order kinetics, while sucrose hydrolysis follows Michaelis-Menten behavior, the hydrolytic activity of S425A is reduced to about 52% of the converted sucrose | Leuconostoc citreum |
S425A | the mutant no longer produces high-molecular-weight inulin | Leuconostoc citreum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
82.6 | - |
sucrose | wild type enzyme, at 30°C and pH 6.0 | Leuconostoc citreum | |
125 | - |
sucrose | mutant enzyme S425A, at 30°C and pH 6.0 | Leuconostoc citreum | |
126 | - |
sucrose | mutant enzyme R618K, at 30°C and pH 6.0 | Leuconostoc citreum | |
308 | - |
sucrose | mutant enzyme L499F, at 30°C and pH 6.0 | Leuconostoc citreum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc citreum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,1-beta-D-fructosyl)n | - |
Leuconostoc citreum | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fructosyltransferase | - |
Leuconostoc citreum |
FTF | - |
Leuconostoc citreum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
the wild type enzyme shows a half-life of 2.2 h at 35°C | Leuconostoc citreum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
sucrose | mutant enzyme S425A, at 30°C and pH 6.0 | Leuconostoc citreum | |
27 | - |
sucrose | mutant enzyme R618K, at 30°C and pH 6.0 | Leuconostoc citreum | |
71 | - |
sucrose | wild type enzyme, at 30°C and pH 6.0 | Leuconostoc citreum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
sucrose | mutant enzyme S425A, at 30°C and pH 6.0 | Leuconostoc citreum | |
0.217 | - |
sucrose | mutant enzyme R618K, at 30°C and pH 6.0 | Leuconostoc citreum | |
0.861 | - |
sucrose | wild type enzyme, at 30°C and pH 6.0 | Leuconostoc citreum |