Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.91 extracted from

  • Sutter, A.; Grisebach, H.
    UDP-glucose: flavonol 3-0-glucosyltransferase from cell suspension cultures of parsley (1973), Biochim. Biophys. Acta, 309, 289-295.
    View publication on PubMed
Search for more literature for 2.4.1.91

Activating Compound

Activating Compound Comment Organism Structure
glycine-HCl glycine-HCl buffer stimulates about 2fold at pH 9 in comparison with Tris-HCl buffer Petroselinum crispum

Inhibitors

Inhibitors Comment Organism Structure
additional information enzyme is inhibited in the crude extract Petroselinum crispum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 quercetin less than 0.001 mM Petroselinum crispum
0.5
-
 UDPglucose
-
 Petroselinum crispum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + a flavonol Petroselinum crispum the enzyme is involved in the flavone glycoside pathway in parsley. Enzyme activity is increased by prior illumination of the cell cultures, maximal activity is reached about 24 h after onset of illumination UDP + a flavonol 3-O-D-glucoside
-
 ?

Organism

Organism UniProt Comment Textmining
Petroselinum crispum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Petroselinum crispum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with dihydroquercetin Petroselinum crispum ?
-
 ?
UDPglucose + 3'-O-methylquercetin 28% of the activity with quercetin Petroselinum crispum UDP + 3'-O-methylquercetin 3-O-glucoside
-
 ?
UDPglucose + 4,7-dihydroxyflavonol 27% of the activity with quercetin Petroselinum crispum UDP + 4,7-dihydroxyflavonol 3-O-glucoside
-
 ?
UDPglucose + 5-deoxyquercetin 57% of the activity with quercetin Petroselinum crispum UDP + 5-deoxyquercetin 3-O-glucoside
-
 ?
UDPglucose + a flavonol the enzyme is involved in the flavone glycoside pathway in parsley. Enzyme activity is increased by prior illumination of the cell cultures, maximal activity is reached about 24 h after onset of illumination Petroselinum crispum UDP + a flavonol 3-O-D-glucoside
-
 ?
UDPglucose + kaempferol 70% of the activity with quercetin Petroselinum crispum UDP + kaempferol 3-O-glucoside
-
 ?
UDPglucose + kaempferol-4'-O-methylether 57% of the activity with quercetin Petroselinum crispum UDP + ?
-
 ?
UDPglucose + quercetin quercetin is the best acceptor Petroselinum crispum UDP + quercetin 3-O-glucoside
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 broad pH-optimum around pH 8 Petroselinum crispum