Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 14 mM, stimulates | Vigna mungo | |
2-mercaptoethanol | 14 mM, activation to 157% | Vigna mungo | |
dithioerythritol | 10 mM, activation to 215% | Vigna mungo | |
EDTA | 1 mM, activation to 173% | Vigna mungo |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 10 mM, 17% inhibition | Vigna mungo | |
CoCl2 | 1 mM, 35% inhibition | Vigna mungo | |
Cu2+ | 1 mM CuCl2, 96% inhibition | Vigna mungo | |
iodoacetate | - |
Vigna mungo | |
KCl | 10 mM, 17% inhibition | Vigna mungo | |
Mg2+ | 1 mM MgCl2, 18% inhibition | Vigna mungo | |
Mn2+ | 10 mM MnCl2, 35% inhibition | Vigna mungo | |
NEM | - |
Vigna mungo | |
PCMB | - |
Vigna mungo | |
Phenylmercuriacetate | - |
Vigna mungo | |
Zn2+ | 1 mM ZnCl2, 96% inhibition | Vigna mungo |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00067 | - |
kaempferol | - |
Vigna mungo | |
0.69 | - |
kaempferol | - |
Vigna mungo | |
1.67 | - |
UDP-D-glucose | - |
Vigna mungo |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
gel filtration | Vigna mungo |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna mungo | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Vigna mungo |
partial | Vigna mungo |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Vigna mungo | - |
Storage Stability | Organism |
---|---|
-20°C, 20 mM imidazole-HCl, pH 8.0, 14 mM 2-mercaptoethanol, 10% glycerol, 35% loss of activity after 2 days, 61% loss of activity after 1 week | Vigna mungo |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + isorhamnetin | - |
Vigna mungo | UDP + isorhamnetin 3-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + isorhamnetin | 108% of the activity with kaempferol | Vigna mungo | UDP + isorhamnetin 3-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + kaempferol 7-O-glucoside | 10% of the activity with kaempferol | Vigna mungo | UDP + kaempferol 3,7-O-diglucoside | - |
? | |
UDP-alpha-D-glucose + quercetin 7-O-beta-D-glucoside | 12% of the activity with kaempferol | Vigna mungo | UDP + quercetin 3,7-O-di-beta-D-glucoside | - |
? | |
UDPglucose + kaempferol | - |
Vigna mungo | UDP + kaempferol 3-O-glucoside | - |
? | |
UDPglucose + kaempferol 5,7,4'-trimethyl ether | 9% of the activity with kaempferol | Vigna mungo | UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside | - |
? | |
UDPglucose + kaempferol-4'-O-methylether | 42% of the activity with kaempferol | Vigna mungo | UDP + ? | - |
? | |
UDPglucose + myricetin | 20% of the activity with kaempferol | Vigna mungo | UDP + myrecetin 3-O-glucoside | - |
? | |
UDPglucose + quercetin | - |
Vigna mungo | UDP + quercetin 3-O-glucoside | - |
? | |
UDPglucose + quercetin | 95% of the activity with quercetin | Vigna mungo | UDP + quercetin 3-O-glucoside | - |
? | |
UDPglucose + quercetin 5-methyl ether | 7% of the activity with kaempferol | Vigna mungo | UDP + quercetin 5-methyl ether 3-O-glucoside | - |
? | |
UDPglucose + rhamnetin | 98% of the activity with kaempferol | Vigna mungo | UDP + rhamnetin 3-O-glucoside | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
in histidine-HCl buffer | Vigna mungo |
8 | - |
in imidazole-HCl buffer | Vigna mungo |