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Literature summary for 2.4.1.96 extracted from
- Purification of UDOgalactose:sn-glycerol-3-phosphate alpha-D-galactosyltransferase from Poterioochromonas malhamensis (1983), Biochim. Biophys. Acta, 759, 154-159.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| cetyltrimethylammonium bromide, optimal concentration for stabilization, 0.075% w/v at pH 8.5, 0.1% w/v at pH 7.2, 0.2 w/v at pH 6.2 | Poterioochromonas malhamensis |
| glycerol, 30% v/v, stabilizes the enzyme at pH 7.8 but not at lower or higher pH values | Poterioochromonas malhamensis |
| unstable to repeated freezing and thawing | Poterioochromonas malhamensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
UDPgalactose | - |
Poterioochromonas malhamensis |  |
| 0.02 | - |
sn-glycerol 3-phosphate | - |
Poterioochromonas malhamensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
gel filtration | Poterioochromonas malhamensis |
| 70000 | - |
1 * 70000, SDS-PAGE | Poterioochromonas malhamensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Poterioochromonas malhamensis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Poterioochromonas malhamensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 198 | - |
- |
Poterioochromonas malhamensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPgalactose + sn-glycerol 3-phosphate | - |
Poterioochromonas malhamensis | UDP + alpha-D-galactosyl sn-glycerol 3-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Poterioochromonas malhamensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Poterioochromonas malhamensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | about 50% of activity maximum at pH 6 and pH 9 | Poterioochromonas malhamensis |
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Release 2023.1 (January 2023)
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