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Literature summary for 2.4.1.99 extracted from
- Purification and characterization of sucrose:sucrose 1-fructosyltransferase from the roots of Asparagus (Asparagus officinalis L.) (1980), Agric. Biol. Chem., 44, 603-614.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 71% inhibition | Asparagus officinalis |  |
| Hg2+ | 0.1 mM, 81% inhibition | Asparagus officinalis | |
| Mn2+ | 1.0 mM, 75% inhibition | Asparagus officinalis | |
| PCMB | 0.12 mM, 22% inhibition | Asparagus officinalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
sucrose | - |
Asparagus officinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
gel filtration | Asparagus officinalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Asparagus officinalis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Asparagus officinalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Asparagus officinalis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.97 | - |
- |
Asparagus officinalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + 6G(1-beta-fructofuranosyl)2-sucrose | - |
Asparagus officinalis | D-glucose + 1F-beta-fructofuranosyl-6G(1-beta-fructofuranosyl)2-sucrose | - |
? | |
| sucrose + neokestose | - |
Asparagus officinalis | D-glucose + 1F,6G-di-beta-fructofuranosylsucrose | - |
? | |
| sucrose + sucrose | - |
Asparagus officinalis | D-glucose + 1F-beta-D-fructosylsucrose | - |
? | |
| sucrose + sucrose | - |
Asparagus officinalis | D-glucose + 1F-beta-D-fructosylsucrose | - |
r | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
pH 4.0-8.0, 30 min, stable | Asparagus officinalis |
| 45 | - |
pH 5.0-6.5, 50% loss of activity after 30 min, pH 4.0 and pH 8.0, more than 90% loss of activity after 30 min | Asparagus officinalis |
| 60 | - |
10 min, 82% loss of activity | Asparagus officinalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Asparagus officinalis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 30°C, stable | Asparagus officinalis |
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