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Literature summary for 2.4.1.99 extracted from

  • Tamura, K.; Kawakami, A.; Sanada, Y.; Tase, K.; Komatsu, T.; Yoshida, M.
    Cloning and functional analysis of a fructosyltransferase cDNA for synthesis of highly polymerized levans in timothy (Phleum pratense L.) (2009), J. Exp. Bot., 60, 893-905.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Phleum pratense

Organism

Organism UniProt Comment Textmining
Phleum pratense
-
 timothy grass
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phleum pratense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose 1-SST activity is about 5times higher than 6-SST activity when the protein is incubated with sucrose for short periods Phleum pratense D-glucose + 1-kestose
-
 ?
sucrose + sucrose FT1 has high affinity for 6-kestose and low affinity for 1-kestose Phleum pratense D-glucose + 6-kestose
-
 ?

Synonyms

Synonyms Comment Organism
1-SST
-
 Phleum pratense
6-SST
-
 Phleum pratense
FT1 FT1 shows invertase, sucrose:fructan 6-fructosyltransferase, and sucrose:sucrose 1-fructosyltransferase activities Phleum pratense
sucrose:sucrose 1-fructosyltransferase
-
 Phleum pratense
sucrose:sucrose 6-fructosyltransferase
-
 Phleum pratense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Phleum pratense

Expression

Organism Comment Expression
Phleum pratense FT1 transcription is readily induced under high sucrose or cold conditions associated with increased sucrose:sucrose 6-fructosyltransferase activity up