Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Lolium perenne |
General Stability | Organism |
---|---|
several attempts to purify 1-SST are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium | Lolium perenne |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
272 | - |
sucrose | wild type enzyme, in 50 mM sodium acetate buffer, pH 5.0, at 30°C | Lolium perenne |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lolium perenne | Q84RM0 | perennial ryegrass | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 1-SST are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium | Lolium perenne |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + sucrose | 1-SST uses sucrose as a preferential donor and acceptor substrate, 1-SST can only create a beta(2->1) linkage between two fructosyl residues | Lolium perenne | D-glucose + 1-kestose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-SST | - |
Lolium perenne |
sucrose:sucrose 1-fructosyltransferase | - |
Lolium perenne |