Cloned (Comment) | Organism |
---|---|
expression of native and E1526A mutant enzyme in Schizosaccharomyces pombe, overexpression of native but not mutant enzyme results in accumulation of (1,4)-alpha-glucan | Schizosaccharomyces pombe |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1118-1689 | enzyme is not able to synthesize alpha-1,4-glucan | Schizosaccharomyces pombe |
DELTA113-724 | enzyme is able to synthesize alpha-1,4-glucan | Schizosaccharomyces pombe |
E1526A | markedly reduced ability to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant, no accumulation of alpha-1,4-glucan | Schizosaccharomyces pombe |
E1534A | able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant | Schizosaccharomyces pombe |
G1165A | able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant | Schizosaccharomyces pombe |
G696S | temperature-sensitive mutation, gene is functional at 28°C but not at 36°C | Schizosaccharomyces pombe |
K1163Q | able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant | Schizosaccharomyces pombe |
K1422Q | reduced accumulation of alpha-1,4-glucan | Schizosaccharomyces pombe |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Schizosaccharomyces pombe | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
265000 | - |
x * 265000, SDS-PAGE | Schizosaccharomyces pombe |
272106 | - |
x * 272106, calculated from the deduced amino acid sequence | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Schizosaccharomyces pombe | required for the biosynthesis of alpha-1,4-glucan in cell wall alpha-glucan | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | Q9USK8 | study deals with the intracellular domain of the protein | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-glucose + [alpha-D-glucosyl-(1-3)]n = UDP + alpha-1,4-glucosyl-[alpha-D-glucosyl-(1-3)]n | adds the approximately 10% of alpha-1,4-linked glucose residues to alpha-1,3-glucan chains | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | required for the biosynthesis of alpha-1,4-glucan in cell wall alpha-glucan | Schizosaccharomyces pombe | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 265000, SDS-PAGE | Schizosaccharomyces pombe |
? | x * 272106, calculated from the deduced amino acid sequence | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
Ags1p | multidomain protein with two probable catalytic domains predicted to reside at opposite sides of the plasma membrane, as well as a multipass transmembrane domain | Schizosaccharomyces pombe |
cell wall alpha-1,4-glucan synthase | - |
Schizosaccharomyces pombe |