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Literature summary for 2.4.1.B52 extracted from
- Higher expression of the strawberry xyloglucan endotransglucosylase/hydrolase genes FvXTH9 and FvXTH6 accelerates fruit ripening (2019), Plant J., 100, 1237-1253 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH9 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH6, recombinant expression in Nicotiana tabacum cv. Samsum Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells | Fragaria vesca |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | Fragaria x ananassa cv. Elsanta fruits infiltrated with FvXTH9 and FvXTH6 ripen faster and show decreased firmness compared with the empty vector control pBI121 | Fragaria vesca |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | prediction of subcellular localization suggests a localization to the secretory pathway | Fragaria vesca | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fragaria vesca | - |
cv. Hawaii 4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Saccharomyces cerevisiae INVSc1 cells by nickel affinity chromatography | Fragaria vesca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | high expression level of FvXTH9 | Fragaria vesca | - |
| fruit | FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages | Fragaria vesca | - |
| additional information | to confirm the transcriptome data, the expression pattern of FvXTH9 is examined by quantitative real-time PCR in fruit at different ripening stages as well as in leaf and flower tissues. FvXTH9 is highly expressed in fully developed green fruit, whereas its expression level dropped in later stages. A high expression level of FvXTH9 is also determined in flowers, whereas its mRNA abundance is very low in all other tissues investigated. The expression pattern of FvXTH6 during fruit development resembled that of FvXTH9. FvXTH9 shows the highest expression level in green receptacles | Fragaria vesca | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| barley (1->3,1->4)-beta-D-glucan + XLLGol | MXE activity | Fragaria vesca | ? | - |
? |  |
| barley (1->3,1->4)-beta-D-glucan + XXFGol | MXE activity | Fragaria vesca | ? | - |
? | |
| barley (1->3,1->4)-beta-D-glucan + XXGol | MXE activity | Fragaria vesca | ? | - |
? | |
| barley (1->3,1->4)-beta-D-glucan + XXLGol | MXE activity | Fragaria vesca | ? | - |
? | |
| barley (1->3,1->4)-beta-D-glucan + XXXGol | MXE activity | Fragaria vesca | ? | - |
? | |
| hydroxyethylcellulose + XXXGol | CXE activity | Fragaria vesca | ? | - |
? | |
| additional information | enzyme FvXTH9 shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE), cellulose:xyloglucan endotransglucosylase (CXE), and xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activities | Fragaria vesca | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 33200, about, sequence calculation | Fragaria vesca |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellulose:xyloglucan endotransglucosylase | - |
Fragaria vesca |
| CXE | - |
Fragaria vesca |
| FvXTH9 | - |
Fragaria vesca |
| mixed-linkage glucan:xyloglucan endotransglucosylase | - |
Fragaria vesca |
| More | see also EC 2.4.1.207 | Fragaria vesca |
| MXE | - |
Fragaria vesca |
| XTH9 | - |
Fragaria vesca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Fragaria vesca |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
CXE activity | Fragaria vesca |
| 6.5 | - |
MXE activity | Fragaria vesca |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fragaria vesca | sequence calculation | - |
5.46 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | XTHs are classified together with the lichenases, which hydrolyse mixed-linkage (1->3,1->4)-beta-D-glucans (MLG), in glycoside hydrolase family 16 | Fragaria vesca |
| metabolism | enzymes FvXTH9 and FvXTH6 display xyloglucan endotransglucosylase (XET, EC 2.4.1.207) activity towards various acceptor substrates using xyloglucan as the donor substrate. FvXTH9 also shows activity of mixed-linkage glucan:xyloglucan endotransglucosylase (MXE) and cellulose:xyloglucan endotransglucosylase (CXE). XTHs (xyloglucan endotransglucosylases/hydrolases) can catalyse the endolytic cleavage of xyloglucan polymers and the rejoining of the newly generated reducing ends to other xyloglucan molecules, which is referred to as xyloglucan endotransglucosylase (XET) activity. In addition, XTHs can also show xyloglucan endohydrolase (XEH) activity, where water is used as an acceptor, and thus the xyloglucan molecule is hydrolysed | Fragaria vesca |
| physiological function | FvXTH9 and also FvXTH6 might promote strawberry fruit ripening by the modification of cell wall components. The level of anthocyanins such as pelargonidin-3-O-glucoside and pelargonidin-3-O-(6'-malonyl)-glucoside is significantly higher in the infiltrated fruits, similar to citric acid and ascorbic acid | Fragaria vesca |
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