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Literature summary for 2.4.1.B62 extracted from

  • Ziegler, M.O.; Jank, T.; Aktories, K.; Schulz, G.E.
    Conformational changes and reaction of clostridial glycosylating toxins (2007), J. Mol. Biol., 377, 1346-1356.
    View publication on PubMed
Search for more literature for 2.4.1.B62

Cloned(Commentary)

Cloned (Comment) Organism
expression of catalytic fragment, residues 1-546 Paeniclostridium sordellii
expression of catalytic fragment, residues 1-551 Clostridium novyi

Crystallization (Commentary)

Crystallization (Comment) Organism
catalytic fragment, residues 1-546, to 2.3 A resolution. Geometry suggests that the reaction runs as a circular electron transfer in a six-membered ring, which involves the deprotonation of the nucleophile by the beta-phosphoryl group of the donor substrate UDP-glucose Paeniclostridium sordellii
catalytic fragment, residues 1-551 to 2.85 A resolution. Geometry suggests that the reaction runs as a circular electron transfer in a six-membered ring, which involves the deprotonation of the nucleophile by the beta-phosphoryl group of the donor substrate UDP-glucose Clostridium novyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63992
-
 x * 63992, catalytic fragment, residues 1-546 Paeniclostridium sordellii
63992
-
 x * 63992, catalytic fragment, residues 1-551 Clostridium novyi

Organism

Organism UniProt Comment Textmining
Clostridium novyi Q46149
-
-
Paeniclostridium sordellii Q46342
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information a circular electron transfer reaction is suggested tha does not directly involve any residues from the toxin. The transfer starts with the split of the glycosidic bond leading to the most stable transient state. The split increases the pK of the phosphoryl oxygen atom, facilitating deprotonation of the accepor, and provides space for the nucleophilic attack Clostridium novyi ?
-
 ?
additional information a circular electron transfer reaction is suggested tha does not directly involve any residues from the toxin. The transfer starts with the split of the glycosidic bond leading to the most stable transient state. The split increases the pK of the phosphoryl oxygen atom, facilitating deprotonation of the accepor, and provides space for the nucleophilic attack Paeniclostridium sordellii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 63992, catalytic fragment, residues 1-546 Paeniclostridium sordellii
? x * 63992, catalytic fragment, residues 1-551 Clostridium novyi