Literature summary for 2.4.1.B64 extracted from

Muheim, C.; Bakali, A.; Engstroem, O.; Wieslander, A.; Daley, D.O.; Widmalm, G.
Identification of a fragment-based scaffold that inhibits the glycosyltransferase WaaG from Escherichia coli (2016), Antibiotics, 5, 10.

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Activating Compound

Activating Compound	Comment	Organism	Structure
3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate	presence of 20 mM in assay, enhances activity	Escherichia coli
cardiolipin	presence of 1 mM in assay, enhances activity	Escherichia coli
phosphatidylglycerol	presence of 10 mM in assay, enhances activity	Escherichia coli

Application

Application	Comment	Organism
analysis	activity assay for WaaG using 14C-labeled UDP-glucose and lipopolysaccharide purified from a WaaG deletion strain of Escherichia coli. Addition of the lipids phosphatidylglycerol and cardiolipin, as well as the detergent 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate increase activity	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
4-(2-amino-1,3-thiazol-4-yl)phenol	-	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	absolutely required, assay in presence of 25 mM MgCl2	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q9R2L8	-	-
Escherichia coli F470	Q9R2L8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose + truncated lipopolysaccharide	i.e. lipopolysaccharide isolated from a WaaG-deletion strain	Escherichia coli	UDP + glucosylated truncated lipopolysaccharide	-	?
UDP-alpha-D-glucose + truncated lipopolysaccharide	i.e. lipopolysaccharide isolated from a WaaG-deletion strain	Escherichia coli F470	UDP + glucosylated truncated lipopolysaccharide	-	?

Synonyms

Synonyms	Comment	Organism
WaaG	-	Escherichia coli

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
1	-	pH 7.5, 22°C	Escherichia coli	4-(2-amino-1,3-thiazol-4-yl)phenol

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Release 2023.1 (January 2023)