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Literature summary for 2.4.2.1 extracted from

  • Surette, M.; Gill, T.; MacLean, S.
    Purification and characterization of purine nucleoside phosphorylase from Proteus vulgaris (1990), Appl. Environ. Microbiol., 56, 1435-1439.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.1

Inhibitors

Inhibitors Comment Organism Structure
adenosine
-
 Proteus vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 phosphate reaction with inosine Proteus vulgaris
0.015
-
 phosphate reaction with guanosine Proteus vulgaris
0.029
-
 guanosine
-
 Proteus vulgaris
0.039
-
 Inosine
-
 Proteus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
120000
-
 non-denaturing PAGE Proteus vulgaris

Organism

Organism UniProt Comment Textmining
Proteus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Proteus vulgaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Proteus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine + phosphate
-
 Proteus vulgaris guanine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Proteus vulgaris hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5 TES buffer Proteus vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
6.8 7.8 pH 6.8: about 70% of maximal activity, pH 7.8: about 40% of maximal activity Proteus vulgaris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.049
-
 adenosine
-
 Proteus vulgaris