Any feedback?
Literature summary for 2.4.2.1 extracted from
- Purification and characterization of thermostable purine nucleoside phosphorylase of Bacillus stearothermophilus JTS 859 (1989), Agric. Biol. Chem., 53, 2205-2210.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| HgCl2 | - |
Geobacillus stearothermophilus |  |
| PCMB | - |
Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
2'-deoxyguanosine | - |
Geobacillus stearothermophilus | |
| 0.14 | - |
guanosine | - |
Geobacillus stearothermophilus | |
| 0.2 | - |
deoxyinosine | - |
Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 34000, SDS-PAGE | Geobacillus stearothermophilus |
| 68000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
JTS 859 | - |
| Geobacillus stearothermophilus JTS 859 | - |
JTS 859 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 405 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyguanosine + phosphate | - |
Geobacillus stearothermophilus | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyguanosine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | guanine + alpha-D-deoxyribose 1-phosphate | - |
? | |
| deoxyinosine + phosphate | - |
Geobacillus stearothermophilus | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| deoxyinosine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | hypoxanthine + alpha-D-deoxyribose 1-phosphate | - |
r | |
| guanosine + phosphate | - |
Geobacillus stearothermophilus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Geobacillus stearothermophilus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Geobacillus stearothermophilus JTS 859 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 34000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 h, stable | Geobacillus stearothermophilus |
| 80 | - |
half-life is 16 h in 20 mM potassium phosphate and 1 mM inosine, pH 7.0 | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 11 | - |
Geobacillus stearothermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 12 | pH 6.0: about 65% of maximal activity, pH 12.0: about 25% of maximal activity | Geobacillus stearothermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
