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Literature summary for 2.4.2.1 extracted from

  • May, R.A.; Hoffee, P.
    Purine nucleoside phosphorylases purified from rat liver and Novikoff hepatoma cells (1979), Arch. Biochem. Biophys., 193, 398-406.
    View publication on PubMed
Search for more literature for 2.4.2.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Inosine
-
 Rattus norvegicus
0.12
-
 guanosine
-
 Rattus norvegicus
0.52
-
 phosphate
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 3 * 29000, SDS-PAGE Rattus norvegicus
84000
-
 gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-
Novikoff hepatoma cell
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
132
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-mercaptopurine riboside + phosphate 14% of the activity with inosine Rattus norvegicus alpha-D-ribose 1-phosphate + 6-mercaptopurine
-
 ?
guanosine + phosphate 16% of the activity with inosine Rattus norvegicus guanine + alpha-D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Rattus norvegicus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

Subunits Comment Organism
trimer 3 * 29000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 7.4, 20 min, about 20% loss of activity Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Rattus norvegicus