Any feedback?
Literature summary for 2.4.2.1 extracted from
- Bovine brain purine-nucleoside phosphorylase purification, characterization, and catalytic mechanism (1976), Biochemistry, 15, 4451-4457.
General Stability
| General Stability | Organism |
|---|---|
| strong susceptibility to photooxidation in presence of methylene blue. Maximal photoinactivation at pH 8.0, 85% loss of activity after only 1 min of irradiation | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-ribose 1-phosphate | - |
Bos taurus |  |
| guanine | - |
Bos taurus | |
| guanosine | - |
Bos taurus | |
| hypoxanthine | - |
Bos taurus | |
| PCMB | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
Inosine | - |
Bos taurus | |
| 0.03 | - |
guanine | - |
Bos taurus | |
| 0.0435 | - |
guanosine | - |
Bos taurus | |
| 21.5 | - |
phosphate | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Bos taurus |
| 78000 | 80000 | gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | Theorell-Chance mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 78.3 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + phosphate | - |
Bos taurus | guanine + alpha-D-ribose 1-phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
rapid inactivation | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
guanine | with guanosine as variable substrate and 200 mM phosphate | Bos taurus | |
| 0.0145 | - |
guanine | with guanosine as variable substrate and 40 mM phosphate | Bos taurus | |
| 0.0313 | - |
guanosine | with guanine as variable substrate and 0.4 mM ribose 1-phosphate | Bos taurus | |
| 0.05 | - |
hypoxanthine | with guanosine as variable substrate and 200 mM phosphate | Bos taurus | |
| 0.065 | - |
guanine | with phosphate as variable substrate and 0.2 mM guanosine | Bos taurus | |
| 0.32 | - |
alpha-D-ribose 1-phosphate | with phosphate as variable substrate and 0.2 mM guanosine | Bos taurus | |
| 0.36 | - |
alpha-D-ribose 1-phosphate | with guanosine as variable substrate and 20 mM phosphate | Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
