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Literature summary for 2.4.2.1 extracted from

  • Murkin, A.S.; Birck, M.R.; Rinaldo-Matthis, A.; Shi, W.; Taylor, E.A.; Almo, S.C.; Schramm, V.L.
    Neighboring group participation in the transition state of human purine nucleoside phosphorylase (2007), Biochemistry, 46, 5038-5049.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
HsPNP/4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H/SO4 crystal complexes are prepared by hanging-drop vapor diffusion, HsPNP/immucillin-H/PO4 crystal complexes are prepared by sitting-drop vapor diffusion Homo sapiens

Protein Variants

Protein Variants Comment Organism
H257D kcat/Km for inosine is 1460fold lower than wild-type value, greatly decreased affinity for Immucillin-H Homo sapiens
H257F kcat/Km for inosine is 68fold lower than wild-type value, greatly decreased affinity for Immucillin-H Homo sapiens
H257G kcat/Km for inosine is 610fold lower than wild-type value, greatly decreased affinity for Immucillin-H Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H
-
 Homo sapiens
5'-deoxy-immucillin-H
-
 Homo sapiens
immucillin-H
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 Inosine 25°C, pH 7.4 Homo sapiens
0.75
-
 Inosine 25°C, pH 7.4 Homo sapiens
1.35
-
 Inosine 25°C, pH 7.4 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P00491
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + phosphate
-
 Homo sapiens hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
HsPNP
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 Inosine 25°C, pH 7.4 Homo sapiens
1.75
-
 Inosine 25°C, pH 7.4 Homo sapiens
6.55
-
 Inosine 25°C, pH 7.4 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000000077
-
 5'-deoxy-immucillin-H 25°C, pH 7.4, mutant enzyme H257G Homo sapiens
0.0000000107
-
 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H 25°C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase Homo sapiens
0.000000011
-
 immucillin-H 25°C, pH 7.4, mutant enzyme H257G Homo sapiens
0.00000002
-
 5'-deoxy-immucillin-H 25°C, pH 7.4, wild-type enzyme Homo sapiens
0.000000054
-
 5'-deoxy-immucillin-H 25°C, pH 7.4, mutant enzyme H257D Homo sapiens
0.0000000579
-
 immucillin-H 25°C, pH 7.4, wild-type enzyme, measured in slow-onset tight-binding phase Homo sapiens
0.000000086
-
 immucillin-H 25°C, pH 7.4, mutant enzyme H257D Homo sapiens
0.000000172
-
 immucillin-H 25°C, pH 7.4, mutant enzyme H257F Homo sapiens
0.000000198
-
 5'-deoxy-immucillin-H 25°C, pH 7.4, mutant enzyme H257F Homo sapiens
0.00000027
-
 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H 25°C, pH 7.4, mutant enzyme H257G, measured in slow-onset tight-binding phase Homo sapiens
0.0000009
-
 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H 25°C, pH 7.4, mutant enzyme H257D, measured in slow-onset tight-binding phase Homo sapiens
0.00000095
-
 4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-H 25°C, pH 7.4, mutant enzyme H257F, measured in slow-onset tight-binding phase Homo sapiens