Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-amino-6-oxo-9-[3-hydroxy-2-(phosphonomethoxy)propyl]-purine | - |
Cellulomonas sp. | |
2-amino-9-[2-(phosphonomethoxy)ethyl]-6-sulfanylpurine | - |
Cellulomonas sp. | |
7-methylguanine | - |
Cellulomonas sp. | |
8-aza-2,6-diamino-(S)-9-[2-(phosphonomethoxy)ethyl]-purine | - |
Cellulomonas sp. | |
alpha-D-ribose 1-phosphate | mixed inhibition of phosphorolysis of inosine | Cellulomonas sp. | |
guanine | competitive inhibition of hydrolysis of inosine | Cellulomonas sp. | |
guanosine | uncompetitive with alpha-D-1-ribose 1-phosphate as varied substrate | Cellulomonas sp. | |
phosphate | - |
Cellulomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0264 | - |
7-methylguanosine | 25°C, pH 7.0 | Cellulomonas sp. | |
0.0343 | - |
phosphate | 25°C, pH 7.0, cosubstrate: 7-methylguanosine | Cellulomonas sp. | |
0.132 | - |
phosphate | 25°C, pH 7.0, cosubstrate: inosine | Cellulomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas sp. | P81989 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
purine ribonucleoside + phosphate = purine + alpha-D-ribose 1-phosphate | mechanism: 1. random binding of substrates, 2. potent binding and slow release of some reaction products leading to the circumstances that the chamical step is not the slowest one and that rapid-equilibrium assumptions do not hold, 3. dual role of phosphate, as substrate and as reaction modifier | Cellulomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylguanosine + phosphate | - |
Cellulomonas sp. | 7-methylguanine + alpha-D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Cellulomonas sp. | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
guanine | 25°C, pH 7.0, with phosphate as varied substrate | Cellulomonas sp. | |
0.0036 | - |
guanine | 25°C, pH 7.0, with inosine as varied substrate | Cellulomonas sp. | |
0.082 | - |
7-methylguanine | 25°C, pH 7.0, with 7-methylguanosine as varied substrate | Cellulomonas sp. | |
0.8 | - |
guanosine | 25°C, pH 7.0, about, with alpha-D-ribose 1-phosphate as varied substrate | Cellulomonas sp. | |
2.5 | - |
phosphate | 25°C, pH 7.0, with alpha-D-ribose 1-phosphate as varied substrate | Cellulomonas sp. | |
4.7 | - |
phosphate | 25°C, pH 7.0, with guanine as varied substrate | Cellulomonas sp. |