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Literature summary for 2.4.2.1 extracted from
- Purine nucleoside phosphorylase from Pseudoalteromonas sp. Bsi590: molecular cloning, gene expression and characterization of the recombinant protein (2008), Extremophiles, 12, 325-333.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Pseudoalteromonas sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
phosphate | - |
Pseudoalteromonas sp. |  |
| 0.32 | - |
Inosine | 37°C | Escherichia coli | |
| 0.34 | - |
phosphate | 37°C | Escherichia coli | |
| 0.67 | - |
Inosine | - |
Pseudoalteromonas sp. | |
| 0.83 | - |
Inosine | 50°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25018 | - |
x * 25018, calculated from sequence | Pseudoalteromonas sp. |
| 29000 | - |
x * 29000, SDS-PAGE | Pseudoalteromonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pseudoalteromonas sp. | A3F8V1 | Bsi590 | - |
| Pseudoalteromonas sp. Bsi590 | A3F8V1 | Bsi590 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudoalteromonas sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-deoxy-6-methylpurine nucleoside + phosphate | 13.2% of the activity with inosine | Pseudoalteromonas sp. | ? | - |
? | |
| 2'-deoxy-6-methylpurine nucleoside + phosphate | 13.2% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | ? | - |
? | |
| 2'-deoxyadenosine + phosphate | 13.4% of the activity with inosine | Pseudoalteromonas sp. | adenine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| 2'-deoxyguanosine + phosphate | 3.7% of the activity with inosine | Pseudoalteromonas sp. | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| 2'-deoxyguanosine + phosphate | 3.7% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | guanine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| adenosine + phosphate | 15.4% of the activity with inosine | Pseudoalteromonas sp. | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| adenosine + phosphate | 15.4% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| arabinofuranosyladenine + phosphate | 4.3% of the activity with inosine | Pseudoalteromonas sp. | ? | - |
? | |
| guanosine + phosphate | 55.8% of the activity with inosine | Pseudoalteromonas sp. | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | 55.8% of the activity with inosine | Pseudoalteromonas sp. Bsi590 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Escherichia coli | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Pseudoalteromonas sp. | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Pseudoalteromonas sp. Bsi590 | hypoxanthine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Pseudoalteromonas sp. |
| ? | x * 25018, calculated from sequence | Pseudoalteromonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EcPNP | - |
Escherichia coli |
| PiPNP | - |
Pseudoalteromonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Pseudoalteromonas sp. |
| 60 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 50 | 15°C: about 45% of maximal activity, 50°C: about 45% of maximal activity | Pseudoalteromonas sp. |
| 25 | 70 | 25°C: about 40% of maximal activity, 70°C: about 60% of maximal activity | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
30 min, stable up to | Pseudoalteromonas sp. |
| 42 | - |
30 min, 26% loss of activity | Pseudoalteromonas sp. |
| 50 | - |
30 min, 15% loss of activity | Escherichia coli |
| 50 | - |
after 1 h of incubation at 50°C with 0.5 M KCl and 1 M KCl, the enzyme activities still retaines 81% and 38%, respectively. It is inactivated without KCl | Pseudoalteromonas sp. |
| 65 | - |
30 min, complete inactivation | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 44.93 | - |
phosphate | 37°C | Escherichia coli | |
| 44.93 | - |
Inosine | 37°C | Escherichia coli | |
| 104.9 | - |
phosphate | 50°C | Escherichia coli | |
| 104.9 | - |
Inosine | 50°C | Escherichia coli | |
| 113.6 | - |
phosphate | - |
Pseudoalteromonas sp. | |
| 113.6 | - |
Inosine | - |
Pseudoalteromonas sp. | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | - |
Pseudoalteromonas sp. |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
stable for 2.5 h | Pseudoalteromonas sp. |
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