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Literature summary for 2.4.2.1 extracted from

  • de Moraes, M.C.; Cardoso, C.L.; Cass, Q.B.
    Immobilized purine nucleoside phosphorylase from Schistosoma mansoni for specific inhibition studies (2013), Anal. Bioanal. Chem., 405, 4871-4878.
    View publication on PubMed
Search for more literature for 2.4.2.1

General Stability

General Stability Organism
immobilization does not affect the catalytic activity and efficiency of the enzyme Schistosoma mansoni

Inhibitors

Inhibitors Comment Organism Structure
2-amino-7-[(3-hydroxyphenyl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one 1.82% inhibition at 0.2 mM Schistosoma mansoni
2-amino-7-[(pyridin-3-yl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one 35.2% inhibition at 0.2 mM Schistosoma mansoni
2-amino-7-[([1,1'-biphenyl]-4-yl)methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one 1.52% inhibition at 0.2 mM Schistosoma mansoni
3-(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-(pyridin-3-yl)propanenitrile 72.7% inhibition at 0.2 mM Schistosoma mansoni
7-benzyl-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one 11% inhibition at 0.2 mM Schistosoma mansoni
phosphate high phosphate concentrations negatively affect the affinity of the enzyme for inosine Schistosoma mansoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 Inosine free enzyme, at pH 7.4, temperature not specified in the publication Schistosoma mansoni
0.164
-
 Inosine immobilized enzyme, at pH 7.4, temperature not specified in the publication Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inosine + phosphate
-
 Schistosoma mansoni hypoxanthine + alpha-D-ribose 1-phosphate
-
 r

Synonyms

Synonyms Comment Organism
PNP
-
 Schistosoma mansoni
purine nucleoside phosphorylase
-
 Schistosoma mansoni

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0075
-
 pH and temperature not specified in the publication Schistosoma mansoni 3-(4-oxo-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)-3-(pyridin-3-yl)propanenitrile