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Literature summary for 2.4.2.1 extracted from
- Purine nucleoside phosphorylase activity decline is linked to the decay of the trimeric form of the enzyme (2014), Arch. Biochem. Biophys., 549, 40-48.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Bos taurus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F159W | the mutant shows reduced activity and stability compared to the wild type enzyme | Bos taurus |
| F200W | the mutant shows reduced activity and stability compared to the wild type enzyme | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31654 | - |
3 * 31654, calculated from amino acid sequence | Bos taurus |
| 32000 | - |
3 * 32000, SDS-PAGE | Bos taurus |
| 100000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| purine deoxyribonucleoside + phosphate | Bos taurus | - |
purine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? |  |
| purine ribonucleoside + phosphate | Bos taurus | - |
purine + alpha-D-ribose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | P55859 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spleen | - |
Bos taurus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 25°C, phosphate buffer pH 7.0, 130 h, 30% loss of activity | Bos taurus |
| 38°C, phosphate buffer pH 7.0, 130 h, 24% loss of activity | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | - |
Bos taurus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| inosine + phosphate | - |
Bos taurus | hypoxanthine + alpha-D-ribose 1-phosphate | - |
r | |
| purine deoxyribonucleoside + phosphate | - |
Bos taurus | purine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? | |
| purine ribonucleoside + phosphate | - |
Bos taurus | purine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotrimer | 3 * 32000, SDS-PAGE | Bos taurus |
| homotrimer | 3 * 31654, calculated from amino acid sequence | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNP | - |
Bos taurus |
| purine nucleoside phosphorylase | - |
Bos taurus |
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Release 2023.1 (January 2023)
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