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Literature summary for 2.4.2.1 extracted from
- Unique substrate specificity of purine nucleoside phosphorylases from Thermus thermophilus (2013), Extremophiles, 17, 505-514.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3) cells | Thermus thermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A196E/D238N | the mutations in the trimeric enzyme form change substrate specificity from guanosine to adenosine | Thermus thermophilus |
| N204D | the mutation in the hexameric enzyme form changes substrate specificity from adenosine to guanosine | Thermus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0071 | - |
guanosine | wild type hexameric enzyme, at pH 8.0 and 70°C | Thermus thermophilus |  |
| 0.083 | - |
adenosine | wild type trimeric enzyme, at pH 8.0 and 70°C | Thermus thermophilus | |
| 0.097 | - |
adenosine | mutant hexameric enzyme N204D, at pH 8.0 and 70°C | Thermus thermophilus | |
| 0.407 | - |
guanosine | mutant trimeric enzyme A196E/D238N, at pH 8.0 and 70°C | Thermus thermophilus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | - |
- |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Toyopearl Phenyl column chromatography, Toyopearl SuperQ column chromatography, and Superdex 200 gel filtration | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| adenosine + phosphate | exclusive substrate for the hexameric enzyme | Thermus thermophilus | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| adenosine + phosphate | exclusive substrate for the hexameric enzyme | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | adenine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | exclusive substrate for the trimeric enzyme | Thermus thermophilus | guanine + alpha-D-ribose 1-phosphate | - |
? | |
| guanosine + phosphate | exclusive substrate for the trimeric enzyme | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | guanine + alpha-D-ribose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer or hexamer | - |
Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PNP | - |
Thermus thermophilus |
| purine nucleoside phosphorylase | - |
Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.22 | - |
adenosine | mutant hexameric enzyme N204D, at pH 8.0 and 70°C | Thermus thermophilus | |
| 0.25 | - |
guanosine | mutant trimeric enzyme A196E/D238N, at pH 8.0 and 70°C | Thermus thermophilus | |
| 0.26 | - |
guanosine | wild type hexameric enzyme, at pH 8.0 and 70°C | Thermus thermophilus | |
| 0.55 | - |
adenosine | wild type trimeric enzyme, at pH 8.0 and 70°C | Thermus thermophilus | |
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