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Literature summary for 2.4.2.1 extracted from

  • Stachelska-Wierzchowska, A.; Wierzchowski, J.; Bzowska, A.; Wielgus-Kutrowska, B.
    Site-selective ribosylation of fluorescent nucleobase analogs using purine-nucleoside phosphorylase as a catalyst: effects of point mutations (2015), Molecules, 21, E44.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 2,6-diamino-8-azapurine mutant enzyme N243D, pH and temperature not specified in the publication Bos taurus
0.06
-
 2,6-diamino-8-azapurine wild type enzyme, pH and temperature not specified in the publication Bos taurus
0.09
-
 8-Azaguanine wild type enzyme, pH and temperature not specified in the publication Bos taurus
0.1
-
 8-Azaguanine Km above 0.1 mM, mutant enzyme N243D, pH and temperature not specified in the publication Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
spleen
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-diamino-8-azapurine + alpha-D-ribose 1-phosphate
-
 Bos taurus N8-ribosyl-2,6-diamino-8-azapurine + N7-ribosyl-2,6-diamino-8-azapurine + phosphate
-
 ?
8-azaguanine + alpha-D-ribose 1-phosphate
-
 Bos taurus N9-beta-D-ribosyl-8-azaguanine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
PNP
-
 Bos taurus