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Literature summary for 2.4.2.10 extracted from
- Orotate phosphoribosyltransferase (yeast) (1978), Methods Enzymol., 51, 69-74.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, complete inhibition | Saccharomyces cerevisiae |  |
| orotidylate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | principles of assay | Saccharomyces cerevisiae | |
| 0.008 | 0.0083 | orotidine 5'-phosphate | - |
Saccharomyces cerevisiae | |
| 0.03 | 0.035 | Orotate | - |
Saccharomyces cerevisiae | |
| 0.062 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | |
| 0.22 | 0.25 | diphosphate | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 81.6 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 7.5-9.5, at least 6 months | Saccharomyces cerevisiae |
| 4°C, gradual loss of activity at pH 7.5-9.5 | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharomyces cerevisiae | orotidine 5'-phosphate + diphosphate | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | at least 6 months stable, -20°C | Saccharomyces cerevisiae |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0063 | - |
orotydilate | plus 5-phospho-alpha-D-ribose 1-diphosphate | Saccharomyces cerevisiae | |
| 0.0083 | - |
orotydilate | plus orotate | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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