Cloned (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
General Stability | Organism |
---|---|
recombinant enzyme, stable for at least 3 months at 4°C or -20°C in presence of 1 mM dithiothreitol and 10% glycerol | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-aminoorotate | 50% inhibition at 0.01 mM | Plasmodium falciparum | |
5-bromoorotate | - |
Plasmodium falciparum | |
5-Fluoroorotate | 50% inhibition at 2-10 nM | Plasmodium falciparum | |
5-fluorouracil | 50% inhibition at 0.005-0.01 mM | Plasmodium falciparum | |
5-iodoorotate | - |
Plasmodium falciparum | |
5-Methylorotate | 50% inhibition at 0.001 mM | Plasmodium falciparum | |
5-phospho-alpha-D-ribose 1-diphosphate | product inhibition, competitive to diphosphate, competitive to orotidine 5'-phosphate | Plasmodium falciparum | |
diphosphate | product inhibition, noncompetitive to orotate, competitive to 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | |
Orotate | product inhibition, noncompetitive to diphosphate, competitive to orotidine 5'-phosphate | Plasmodium falciparum | |
orotidine 5'-phosphate | product inhibition, competitive to 5-phospho-alpha-D-ribose 1-diphosphate, competitive to orotate | Plasmodium falciparum | |
pyrazofurin | 50% inhibition at 0.006-0.024 mM | Plasmodium falciparum | |
Uracil | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0143 | - |
diphosphate | pH 8.0, 37°C | Plasmodium falciparum | |
0.0182 | - |
Orotate | pH 8.0, 37°C | Plasmodium falciparum | |
0.025 | - |
5-Fluoroorotate | pH 8.0, 37°C | Plasmodium falciparum | |
0.0251 | - |
orotidine 5'-phosphate | pH 8.0, 37°C | Plasmodium falciparum | |
0.0282 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 8.0, 37°C | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated | Plasmodium falciparum |
33400 | - |
2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated | Plasmodium falciparum |
67000 | - |
gel filtration, recombinant enzyme | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q8N0R1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Plasmodium falciparum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate | random sequential kinetic mechanism | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluoroorotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | ? | - |
? | |
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | orotidine 5'-phosphate + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 33400, recombinant enzyme, SDS-PAGE, 2 * 33000, calculated | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
diphosphate | pH 8.0, 37°C | Plasmodium falciparum | |
2.6 | - |
orotidine 5'-phosphate | pH 8.0, 37°C | Plasmodium falciparum | |
3 | - |
Orotate | pH 8.0, 37°C | Plasmodium falciparum | |
3.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 8.0, 37°C | Plasmodium falciparum | |
4 | - |
5-Fluoroorotate | pH 8.0, 37°C | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0075 | - |
Orotate | pH 8.0, 37°C, cosubstrate orotidine 5'-phosphate | Plasmodium falciparum | |
0.0153 | - |
5-iodoorotate | pH 8.0, 37°C | Plasmodium falciparum | |
0.0157 | - |
orotidine 5'-phosphate | pH 8.0, 37°C, cosubstrate 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | |
0.0213 | - |
Orotate | pH 8.0, 37°C, cosubstrate diphosphate | Plasmodium falciparum | |
0.0214 | - |
5-Methylorotate | pH 8.0, 37°C | Plasmodium falciparum | |
0.0241 | - |
orotidine 5'-phosphate | pH 8.0, 37°C, cosubstrate orotate | Plasmodium falciparum | |
0.0423 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 8.0, 37°C, cosubstrate diphosphate | Plasmodium falciparum | |
0.0482 | - |
5-bromoorotate | pH 8.0, 37°C | Plasmodium falciparum | |
0.0561 | - |
5-fluorouracil | pH 8.0, 37°C | Plasmodium falciparum | |
0.0705 | - |
pyrazofurin | pH 8.0, 37°C | Plasmodium falciparum | |
0.0797 | - |
diphosphate | pH 8.0, 37°C, cosubstrate orotate | Plasmodium falciparum | |
0.0954 | - |
5-Fluoroorotate | pH 8.0, 37°C | Plasmodium falciparum | |
0.1212 | - |
Uracil | pH 8.0, 37°C | Plasmodium falciparum | |
0.125 | - |
diphosphate | pH 8.0, 37°C, cosubstrate 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | |
0.1501 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 8.0, 37°C, cosubstrate orotidine 5'-phosphate | Plasmodium falciparum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Plasmodium falciparum | calculated | - |
7.1 |