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Literature summary for 2.4.2.10 extracted from

  • Tsutani, Y.; Yoshida, K.; Sanada, Y.; Wada, Y.; Konishi, K.; Fukushima, M.; Okada, M.
    Decreased orotate phosphoribosyltransferase activity produces 5-fluorouracil resistance in a human gastric cancer cell line (2008), Oncol. Rep., 20, 1545-1551.
    View publication on PubMed
Search for more literature for 2.4.2.10

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 Western blot analyis Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
MKN-45 cell parent gastric cancer cell line Homo sapiens
-
additional information MKN-45/F2R cell, 5-fluorouracil resistant gastric cancer cell line Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens 5-fluorouridine 5'-phosphate + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
OPRT
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens mRNA expression of OPRT is markedly decreased to 12.4% and activity to 46.9% in the 5-fluorouracil resistant MKN-45/F2R cell line compared to the MKN-45 parent cell line. OPRT gene expression is markedly decreased to 14.4% when down-regulated by siRNA down

General Information

General Information Comment Organism
malfunction decreased activity of OPRT plays an important role in the acquired resistance of gastric cancer cells towards 5-fluorouracil. OPRT-knockout MKN-45 parent cells using small interfering RNA demonstrate 15.8fold increased resistance to 5-fluorouracil compared to the control cell Homo sapiens