Literature summary for 2.4.2.12 extracted from

van der Veer, E.; Ho, C.; ONeil, C.; Barbosa, N.; Scott, R.; Cregan, S.P.; Pickering, J.G.
Extension of human cell lifespan by nicotinamide phosphoribosyltransferase (2007), J. Biol. Chem., 282, 10841-10845.

Search for more literature for 2.4.2.12

Application

Application	Comment	Organism
medicine	longevity enzyme for human SMCs	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
a retroviral gene delivery system is used to generate human cells stably overexpressing Nampt. Introducing the Nampt gene into aging human SMCs delays senescence and substantially lengthened cell lifespan, together with enhanced resistance to oxidative stress	Homo sapiens
in pQCXIP-IHRES-PURO for retroviral delivery and stable overexpression in Phoenix-amphotropic retrovirus packaging cell line	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
FK866	Nampt antagonist, 10 nM, reduction of Nampt activity in SMCs to 22 +/-2% of baseline activity accompanied with shortened senescent-free survival; reducing Nampt activity with the antagonist FK866 induces premature senescence in smooth muscle cells	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Homo sapiens	rate-limiting enzyme for NAD+ salvage from nicotinamide. Replicative senescence of smooth muscle cells is preceded by a marked decline in the expression and activity of Nampt. Nampt is a longevity protein that can add stress-resistant life to human smooth muscle cells by optimizing SIRT1-mediated p53 degradation	?	-	?
nicotinamide + alpha-D-5-phosphoribosyl 1-diphosphate	Homo sapiens	-	nicotinamide mononucleotide + diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Homo sapiens	P43490	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
aorta thoracica smooth muscle	primary SMC culture stably expressing Nampt, 2.1 +/-0.3 additional population doublings constituting 34 +/-4% lifespan prolongation	Homo sapiens	-
aorta thoracica smooth muscle	primary SMC culture, Nampt expression declines as SMC approach senescence as detected by immunoblotting using rabbit-polyclonal anti-human Nampt/Pre-B-cell colony-enhancing factor antibody	Homo sapiens	-
HITC6 cell	SMC clonal line stably expressing Nampt, 7.1 +/-3.1 fold higher Nampt activity, 6.3 +/-0.3 additional population doublings constituting 71 +/-7% lifespan prolongation, 19 +/-2% reduction in number of senescent cells at passage 37 compared to control HITC6 cells is dependent on SIRT1 activity and associated with increased p53 degradation and protection against oxidative cell damage as response to 150 microM hydrogen peroxide	Homo sapiens	-
HITC6 cell	SMC clonal line, Nampt expression declines as SMC approach senescence as detected by immunoblotting using rabbit-polyclonal anti-human Nampt/Pre-B-cell colony-enhancing factor antibody, 14 +/-3% of basal Nampt activity in presenescent SMC, direct relationship between Nampt activity and number of replication cycles preceeding senescence	Homo sapiens	-
skin fibroblast	from individual with Hutchison-Gilford progeria syndrome associated with premature atherosclerosis, cell lifespan extension upon Nampt overexpression	Homo sapiens	-
vascular smooth muscle cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	rate-limiting enzyme for NAD+ salvage from nicotinamide. Replicative senescence of smooth muscle cells is preceded by a marked decline in the expression and activity of Nampt. Nampt is a longevity protein that can add stress-resistant life to human smooth muscle cells by optimizing SIRT1-mediated p53 degradation	Homo sapiens	?	-	?
nicotinamide + alpha-D-5-phosphoribosyl 1-diphosphate	-	Homo sapiens	nicotinamide mononucleotide + diphosphate	-	r
nicotinamide + alpha-D-5-phosphoribosyl 1-diphosphate	whole cell lysates incubated with 5 microM [carbonyl-14C]nicotinamide + 0.5 mM alpha-D-5-phosphoribosyl-1-diphosphate at pH 8.8	Homo sapiens	nicotinamide mononucleotide + diphosphate	produced NMN quantified by scintillation counting	r

Synonyms

Synonyms	Comment	Organism
Nampt	-	Homo sapiens
Nampt/PBEF/Visfatin	-	Homo sapiens
nicotinamide phosphoribosyltransferase	-	Homo sapiens
PBEF	-	Homo sapiens
pre-B-cell colony-enhancing factor	-	Homo sapiens
Visfatin	-	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)