Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | complex allosteric enzyme whose activity is regulated by a series of conformational changes induced by a number of ligands | Columba livia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Columba livia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
the smaller molecular weight form of the liver enzyme is observed when incubated with purine nucleotides, the smaller one when incubated with phosphoribosyldiphosphate | Columba livia |
102000 | - |
sedimentation equilibrium centrifugation, phosphate, phosphoribosyldiphosphate or purine mononucleotides influence the sedimentation profile | Columba livia |
172000 | - |
sedimentation equilibrium centrifugation in the presence of phosphoribosyldiphosphate, the sedimentation profile is influenced by phosphate, phosphoribosyldiphosphate or purine mononucleotides | Columba livia |
181000 | - |
sedimentation equilibrium centrifugation in the presence of phosphate, the sedimentation profile is influenced by phosphate, phosphoribosyldiphosphate or purine mononucleotides | Columba livia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Columba livia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Columba livia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Columba livia | - |
Storage Stability | Organism |
---|---|
2-mercaptoethanol and phosphoribosyldiphosphate stabilize during storage at 4°C | Columba livia |
4°C, in phosphate buffer, pH 7.4, 60 mM 2-mercaptoethanol and phosphoribosyldiphosphate, at least 10 days | Columba livia |
phosphate stabilizes during storage | Columba livia |
storage in 25 mM Tris buffer inactivates with 26% or 4% residual activity after 6 or 10 days, respectively | Columba livia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Columba livia | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? |