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Literature summary for 2.4.2.14 extracted from

  • Itoh, R.; Holmes, E.W.; Wyngaarden, J.B.
    Pigeon liver amidophosphoribosyltransferase. Ligand-induced alterations in molecular and kinetic properties (1976), J. Biol. Chem., 251, 2234-2240.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information complex allosteric enzyme whose activity is regulated by a series of conformational changes induced by a number of ligands Columba livia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic study Columba livia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
the smaller molecular weight form of the liver enzyme is observed when incubated with purine nucleotides, the smaller one when incubated with phosphoribosyldiphosphate Columba livia
102000
-
sedimentation equilibrium centrifugation, phosphate, phosphoribosyldiphosphate or purine mononucleotides influence the sedimentation profile Columba livia
172000
-
sedimentation equilibrium centrifugation in the presence of phosphoribosyldiphosphate, the sedimentation profile is influenced by phosphate, phosphoribosyldiphosphate or purine mononucleotides Columba livia
181000
-
sedimentation equilibrium centrifugation in the presence of phosphate, the sedimentation profile is influenced by phosphate, phosphoribosyldiphosphate or purine mononucleotides Columba livia

Organism

Organism UniProt Comment Textmining
Columba livia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Columba livia

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Columba livia
-

Storage Stability

Storage Stability Organism
2-mercaptoethanol and phosphoribosyldiphosphate stabilize during storage at 4°C Columba livia
4°C, in phosphate buffer, pH 7.4, 60 mM 2-mercaptoethanol and phosphoribosyldiphosphate, at least 10 days Columba livia
phosphate stabilizes during storage Columba livia
storage in 25 mM Tris buffer inactivates with 26% or 4% residual activity after 6 or 10 days, respectively Columba livia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
Columba livia 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
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