General Stability | Organism |
---|---|
Mg2+ and phosphate are essential for stability | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-iodopurine | 5 mM, 39% inhibition | Homo sapiens | |
6-mercaptopurine ribonucleotide | 5 mM, 64% inhibition | Homo sapiens | |
ADP | 5 mM, 40% inhibition | Homo sapiens | |
allopurinol ribonucleotide | 5 mM, 68% inhibition | Homo sapiens | |
AMP | 5 mM, 76% inhibition, noncompetive vs. glutamine | Homo sapiens | |
ATP | 5 mM, 21% inhibition | Homo sapiens | |
CMP | 5 mM, 22% inhibition | Homo sapiens | |
dCMP | 5 mM, 26% inhibition | Homo sapiens | |
GDP | 5 mM, 40% inhibition | Homo sapiens | |
GMP | 0.5 mM; 5 mM, 70% inhibition | Homo sapiens | |
GTP | 5 mM, 13% inhibition | Homo sapiens | |
IDP | 5 mM, 29% inhibition | Homo sapiens | |
IMP | 5 mM, 41% inhibition | Homo sapiens | |
methyl-dCMP | 5 mM, 47% inhibition | Homo sapiens | |
OMP | 5 mM, 25% inhibition | Homo sapiens | |
phosphate | competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | |
TMP | 5 mM, 42% inhibition | Homo sapiens | |
TTP | 5 mM, 12% inhibition | Homo sapiens | |
UDP | 5 mM, 19% inhibition | Homo sapiens | |
UMP | 5 mM, 25% inhibition | Homo sapiens | |
UTP | 5 mM, 16% inhibition | Homo sapiens | |
XDP | 5 mM, 27% inhibition | Homo sapiens | |
XMP | 5 mM, 41% inhibition | Homo sapiens | |
XTP | 5 mM, 44% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | effect of AMP on kinetic parameters | Homo sapiens | |
0.48 | - |
5-phospho-alpha-D-ribose 1-diphosphate | in Tris buffer | Homo sapiens | |
0.65 | - |
Mg2+ | - |
Homo sapiens | |
1 | 4.5 | L-glutamine | - |
Homo sapiens | |
1.6 | - |
L-glutamine | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | little or no activity in mitochondria or microsomes | Homo sapiens | 5829 | - |
soluble | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activation, approx. 15% as effective as Mg2+ or Mn2+ | Homo sapiens | |
Co2+ | activation, approx. 40% as effective as Mg2+ or Mn2+ | Homo sapiens | |
Mg2+ | required | Homo sapiens | |
Mg2+ | Km-value: 0.65 mM, inhibition above 10 mM | Homo sapiens | |
Mn2+ | required, equally effective as Mg2+ | Homo sapiens | |
additional information | not activated by Ba2+, Cd2+, Cu2+, Fe2+, Hg2+, Ni2+, Zn2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | Homo sapiens | regulating enzyme of purine biosynthesis | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0055 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
4°C, 50 mM phosphate bufer, pH 7.4, 5 mM MgCl2, 60 mM 2-mercaptoethanol, at least 4 weeks, no loss of activity | Homo sapiens |
4°C, partially purified preparation in 50 mM phosphate buffer, pH 7.4, 5 mM Mg2+ and 60 mM 2-mercaptoethanol, at least 4 weeks | Homo sapiens |
4°C, phosphate buffer, 10 d, 40% loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Homo sapiens | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? | |
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | regulating enzyme of purine biosynthesis | Homo sapiens | L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | broad, phosphate buffer | Homo sapiens |
6.5 | - |
imidazole/HCl buffer | Homo sapiens |
7.5 | - |
Tris/HCl buffer | Homo sapiens |