Crystallization (Comment) | Organism |
---|---|
vapour diffusion method, trigonal prisms are obtained using 1.3 M sodium tartrate, 50-200 mM magnesium chloride, 100 mM citrate buffer pH 5.6 and enzyme in the presence of 2 mM AMP, round shaped crystals are obtained with 1.36-1.44 M ammonium sulfate, 0-0.3 M sodium chloride, 100 mM HEPES buffer pH 7.5 and enzyme in the presence of 2 mM AMP | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-histidine | feed-back inhibition | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33367 | - |
6 * 33367, dimers arranged in a hexamer, in the presence of AMP | Escherichia coli |
186000 | - |
dynamic light scattering in the presence of AMP or histidine | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Escherichia coli | first step in histidine biosynthesis | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
centrifugation and DEAE-Sephacel anion-exchange column | Escherichia coli |
Storage Stability | Organism |
---|---|
-20°C, 50 mM Tris-HCl, pH 7.5, 0.4 mM DTT and one protease inhibitor per litre, 50% v/v glycerol, long term storage | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 1-(5-phospho-D-ribosyl)-ATP + diphosphate | - |
? | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | first step in histidine biosynthesis | Escherichia coli | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 33367, dimers arranged in a hexamer, in the presence of AMP | Escherichia coli |